Serum zinc and copper level bofore and after haemodialysis

Zinc and Copper are those trace elements which are necessary for human growth and biological functions. Concentration changes of these elements in the body is clinically important. This research has been set up to study the possibility of zinc and copper variations after haemodialysis, and to determine the serum zinc and copper concentrations before and after haemodialysis. This project is a comparative, non-experimental study. The sample population were 50 of haemodialized patients chosen randomly from the patients referred to Azar hospital in Gorgan in the year of 2003 [33 male and 17 female]. The concentration of serum urea, creatinine, and copper of these patients were determined before and after dialysis, and were analyzed by SPSS software using the Wilkacson tests. The results of this research indicate that the average concentration of zinc and copper in haemodialized patients after dialysis were 135.32, 59.32 and 136.40, 51.25 microgram/deci litre respectively which is markedly increased in comparison to the serum concentrations of these trace elements before dialysis which were 78.38, 37.46 and 89.92, 32.54 microgram/deci litre respectively. In this study the average increase in concentration of zinc and copper after the process of dialysis among the haemodialized patients had a meaningful statistical variation [P<0.001]. Uremia, quality of dialysis membrane and quality of water used for the process of dialysis could be a reason for increase in concentration of zinc and copper

[Effect of hemodialysis on plasma Lipid peroxidation and erythrocyte antioxidant enzyme in Gorgan]

Background and Objective: free radicals are formed in all living organisms during normal cell metabolism. Patients with chronic renal failure, which regularly dialyzed are the candidates for, free radical damages. The aim of this study with the discriminative information was to evaluate the effect of hemodialysis on Lipid peroxidation [The level of Lipid peroxidation expressed as malondialdehyde] and erythrocyte antioxidant enzyme [Glutathione peroxidase] before and after the dialysis and compared with control group, to find out the effect of hemodialysis on the level of Lipid peroxidation of plasma and the activity of erythrocyte antioxidant enzyme

Materials and Methods: this investigation was an analytical type of study and sampling procedure was according to purposive method. 22 patients with chronic renal failure [CRF] disease who were hemodialysed at 5th Azar Hospital of Gorgan dialysis center and 22 age and sex matched healthy control were recruited for this study. The data was analyzed by SPSS software using T-test

Results: plasma malondialdehyde showed significant difference between the predialysis [And control group. It was increased in the postdialysis group [2.32+/-0.38 nmol/ml] when compared with predialysis [1.27+/-0.23 nmol/ml] and control group [0.98+/-0.17 nmol/ml]. Erythrocyte antioxidant enzyme was decreased in postdialysis group [22.26+/-4.76 unit/gram hemoglobin] when compared with predialysis [29.66+/-5.95 unit/gram hemoglobin] and control group [37.52+/-6.26 unit/gram hemoglobin]. There was also significant difference between control and predialysis group. Erythrocyte antioxidant enzyme was lower than control group in dialysis group

Conclusion: the observation of meaningful differences in reduction of erythrocyte antioxidant enzyme and increasing level of plasma Lipid peroxidation in the hemodialysed patients after the process of dialysis, maybe related with the patient, uremia, dialysis membrane [The loosing antioxidant enzyme through this membrane], and the dialysis process [May increase Lipid peroxidation during the dialysis process]. These states of affairs may play an important role in progress of cardiovascular abnormality in hemodialysed patients. Due to this conditions a review of hemodialysis membrane, the techniques used in the dialysis, the consumption of various oral antioxidant, the elimination of active oxygens from the dialysis surrounding are among the measures which can prevent sudden cardiovascular abnormality in the hemodialysis patients and ultimately these important factors up-grade the patients quality of life