ABSTRACT
Diabetes mellitus is common endocrine disease cause learning and memory impairment. This study was done to evaluate the effect of quercetin on learning and memory in STZ-induced diabetic rats was investigated. In this experimental study, 40 male Wistar rats were randomly allocated into five groups: control, quercetin - treated control, diabetic and quercetin - treated diabetic [10 and 20 mg/kg/bw, intraperitoneally] for 14 days. Induction of diabetes was performed using 60 mg/kg/bw of streptozotosin, interapritonally. Passive avoidance and Y-maze tests were used for the evaluation of learning and memory. In passive avoidance learning, there was no significant difference in initial latency between diabetic and treated - diabetic groups. The mean of step latency in control group [383.57 +/- 19.26] significantly reduced to 128.86+/-10.38 in diabetic group [P<0.05]. The mean of step latency in the treated diabetic group significantly increased in compare to the diabetic group [P<0.05]. Step latency in quercetin - treated diabetic [10 mg/kg/bw] and [20 mg/kg/bw] groups increased to 316.67 +/- 23.76 and 397.50+/-31.21, respectively. The alternative percentage in diabetic group was significantly lower than control group [P<0.05], but in quercetin -treated diabetic groups it was higher than the diabetic group [P<0.05]. Administration of quercetin for 14 days enhances the capability of the memory storage, recall and improves short-term spatial memory in STZ-induced diabetic rats
ABSTRACT
Considering the increasing significance of diseases due to NTM all over the world, we investigated the burden of such diseases in our region. The aim of this study was to assess NTM prevalence from different clinical samples during a period of 8 years in Massih Daneshvari Hospital, in Iran. This descriptive study was performed on 8322 samples obtained from pulmonary TB patients in Mycobacteriology Research Center from 2004 -2012. Using Tb1 and Tb2 primers, a 190 bp fragment of IS6110 gene was amplified in order to identify Mycobacterium species. Specimens with negative IS6110 PCR results were analyzed with PCR-RFLP using hsp65 gene, for NTM investigation. Out of 8322 samples, we identified 124 [1.5%] strains of NTM. The mean age of the patients was 57 +/- 18/9 years [age range: 7 - 88 years]. 55/6% of the patients were male. The most common species detected in our study were Mycobacterium simiae [44.3%], Mycobacterium chelonae [16.9%] and Mycobacterium kansasii [12.9%]. We found a high prevalence rate of Mycobacterium simiae among our patients. Treatment protocols for NTM are different from the protocols for Mycobacterium tuberculosis complex, so early diagnosis of these species will be of great importance
ABSTRACT
In recent decades, epidemiology has significantly been considered in hygienic studies and disease control, and has made a way into all the programs and hygiene policies. By examining the convergence of harmful lineage genetic patterns, the common infectious resources among the patients can be inferred. The purpose of this study was to compare the Mycobacterium Tuberculosis genetic patterns convergence isolated from patients infected with Mycobacterium Tuberculosis by MIRU-VNTR technique. After isolation the samples from Lowenstein Jensen culture environment and taking segregate tests and drug susceptibility, the DNA was extracted using CTAB/Nacl technique. The genetic patterns of lineages were calculated according to 12 loci format with MIRU-VNTR technique. Demographic and molecular information of patients was used for epidemiological purposes. After performing drug sensitivity test, 65/140 [64/4%] samples fall into MDR, 29 [20/7%] samples in non MDR category, and the rest of them were among drug. sensitive lineages. Lineage genetic pattern analysis indicated that 49 [35%] of samples related to Delhi/CAS, 28 [20%] to Uganda I, 16 [11/4%] to New I, 1 [0.7%] to EAI, 3[2/1%] to Haarlem, and 5[3/5%] to H37RV families. The genetic pattern convergence comparison exhibited that the most common and variant genetic patterns was seen in Tehran province which were mostly connected to south [from the South of Tehran to Azadi Square] and to the border cities neighboring Afghanistan, Iraq, Turkmenistan and cities with extreme percentage of immigration, all of which signified shared polluted resources among patients
ABSTRACT
Successful implementation of TQM requires a committed leadership, customer - based approaches, process management, efficient communication, knowledge management, empowerment and involvement of staff and an organizational culture for continuous quality improvement. The aim of this study was to assess basic requirements for implementation TQM in Centers of Emergency Medical Services in Iran according EFQM model. In this descriptive study, all of operational and official stations of Medical Emergency services in Ilam, Bushehr, Mazandaran, Ardebil, West Azerbaijan and Yazd province, one half of operational stations in city of Tehran, Sistan-Baluchestan, Khorasan-razavi and Isfahan, were included. All senior managers and personnel of these centers and a random sample of 3246 customers who have received care during the last 6 months were selected. By using a 189 -item's rating scale and 4 questionnaires; leadership style, job and patients satisfaction and state of organizational empowering, according to 5 enabler criteria, from EFQM model was assessed. Validity of instruments by measuring content validity and reliability by internal consistency and test-retest confirmed. Descriptive and inferential statistics used for analyzing data. The overall mean score was 168.4 +/- 34.6 out of 500; therefore, the selected divisions were labeled as drifters organizations which were in early stage of quality improvement road. In comparison, the Tehran, Isfahan and Khorasan-razavi centers with more score labeled as improver's organizations. At the entire, leadership criterion obtained minimum [26.6%] and people criterion obtained maximum [39%] mean scores. Results showed that enabler criteria of the EMS centers in Iran need to reinforcement. It is essential enhancing the knowledge and attitude of managers and personnel about quality improvement principles. Additionally, establishing the management by process, creating customer focused approach and culture of continuous improvement, empowering employees and constituting quality circles are appropriate interventions that should be considered
ABSTRACT
In recent decades, epidemiology has significantly been considered in hygienic studies and disease control, and has made a way into all the programs and hygiene policies. By examining the convergence of harmful lineage genetic patterns, the common infectious resources among the patients can be inferred. The purpose of this study was to compare the Mycobacterium tuberculosis genetic patterns convergence isolated from patients infected with Mycobacterium Tuberculosis by MIRU-VNTR technique. After isolation the samples from Lowenstein Jensen culture environment and taking segregate tests and drug susceptibility, the DNA was extracted using CTAB/NaCl technique. The genetic patterns of lineages were calculated according to 12 loci format with MIRU-VNTR technique. Demographic and molecular information of patients was used for epidemiological purposes. After performing drug sensitivity test, 65/140 [64/4%] samples fall into MDR, 29 [20/7%] samples in non MDR category, and the rest of them were among drug - sensitive lineages. Lineage genetic pattern analysis indicated that 49 [35%] of samples related to Delhi/CAS, 28 [20%] to Uganda I, 16 [11/4%] to New I, 1 [0.7%] to EAI, 3[2/1%] to Haarlem, and 5[3/5%] to H37RV families. The genetic pattern convergence comparison exhibited that the most common and variant genetic patterns was seen in Tehran province which were mostly connected to south [from the South of Tehran to Azadi Square] and to the border cities neighboring Afghanistan, Iraq, Turkmenistan and cities with extreme percentage of immigration, all of which signified shared polluted resources among patients
Subject(s)
Humans , Tuberculosis , Minisatellite Repeats , Interspersed Repetitive Sequences , Drug Resistance, MultipleABSTRACT
The need for recrimping precrimped stainless steel crowns by the dentist in clinic is controversial. This study aimed to evaluate the rate of marginal circumference and marginal thickness change of precrimped stainless steel crowns after recrimping. In this experimental study, 30 primary photos were taken from margins of 30 S.S.Cs [3M, Ni-Cr] related to tooth 85 with a digital camera fixed at a determined distance. Margins of crowns were crimped by 114 and 137 pliers with a controlled force [0.2 N] and then 30 secondary photos were taken in the same conditions. The circumference of crown margins in primary [group A] and secondary [group B] photos were assessed by a digitizer system. Comparing the circumferences of crown margins in primary and secondary photos showed a significant decrease after crimping. Thickness of 30 random points on the crown margins of a crown similar to mentioned cases was measured by SEM [x150]. Then similar procedures including taking a primary photo, crimping and taking a secondary photo was done for the sample crown. After significant reduction in margin circumference, thickness of 30 other random points on the crown margin were measured by SEM. Data were analyzed by paired sample t-test with p<0.05 as the limit of significance. The mean marginal circumference of precrimped stainless steel crowns was reduced by 7.3% which was significant [P<0.001]. On the other hand the mean marginal thickness of sample stainless steel crown showed 18 p. increase. According to the results of this study, marginal circumference of precrimped stainless steel crowns [3M, Ni-Cr] showed a significant decrease after crimping. It is concluded that crimping the stainless steel crowns even for precrimped ones seems necessary