ABSTRACT
The risk of coronary heart disease [CHD] is proportional to the LDL-C lipoprotein. Due to frequent use of Friedwald formula in estimation of LDL-C in most laboratories, this study was done to compare the Friedwald formula and direct measurement to determine the serum levels of LDL-C. This descriptive study was conducted on of 598 patients 226 male and 372 female whome referred to Imam Ali hospital Andimeshk cityin Khozestan province of Iran for health check up during 2009. 5 ml of the venous blood was drown. Total cholesterol [TC] [mg/dl], Triglyceride [TG] [mg/dl], HDL-C [mg/dl] and LDL-C [mg/dl] of serum are measured with Pars azmun company kits. The Friedwald formula was used for estimation of LDL-C. The K=3, 3.5 and 5 were used to stimate the lipid by Friedwald formula. Data were analyzed using SPSS-18, Pearson correlation coefficient and ANOVA tests. A total of 598 serum samples collected; 37.8% were men and 62.2% women. The mean age of participants was 38.8 +/- 10.77 years. Minimum age 21 years and maximum age was 77 years. Mean deviation for TG
ABSTRACT
Snake venom particulary those belonging to Crotalidae and viperidae families, are known to contain number of components affecting blood coagulation. The Aim of this paper is to describe the isolation a specific blood coagulation factor X activator from the crude venom of Iranian Vipera lebetina. Factor X activator was purified from two hundred mg of crude venom by gel filtration on sephadex G-100 and two step ion-exchange chromatography on DEAE-cellulose [DEAE-52]. It showed a single band in sodium dodecyl sulfate-polyacrylamid gel electrophoresis [SDS-PAGE] in non-reducing condition. The mol. wt was estimated to be 78000Da by SDS-PAGE the activator, activated factor X to Xa in presence of calcium ions. It could not activate prothrombin, no had effect an fibrinogen and thus appeared to act specifically on factor X. The activator no amidolytic activtiy on factor Xa substrate benzoyl-Ile-glu-Gly-Arg-p-nitracnilide [S-2222]. It had no arginine esterase activity toward substrate benzoylagrinine ethylester [BAEE] while crude venom hydrolyzes this substrate. The results of this study showed that The activator do act specifically on factor X