Diversity of caprine and ovine Pasteurella multocida isolates based on 16S rRNA gene sequencing

In this study, to increase information about the relationship between caprine and ovine isolates of Pasteurella multocida, 16S rRNA gene sequencing of 9 goats [5] and sheep [4] isolates were investigated. Also, capsular type and toxA gene presentation was studied in this paper. All isolates, except one, belong to capsular type A, and toxA+ strain distributed among strains were isolated from both species. Comparison of the 16S rRNA gene sequence showed the role of goat as a reservoir for P. multocida to sheep independent of toxA genes transmission

Cytoplasmic expression of recombinant interleukin-2 and interleukin-4 proteins results in hydrogen peroxide accumulation and reduction in catalase activity in escherichia coil

The Reactive oxygen species [ROS] is induced in the cells following various stresses but the effect of recombinant protein expression on ROS generation has not been studied yet. In this study, H2O2 concentration and catalase activity variations and their correlation with cell growth following cytoplasmic expression of human interleukin-2 [hIL-2] and mouse interleukin-4 [mIL-4] in Escherichia coli were investigated. Additionally, the effect of recombinant protein expression under different conditions was compared to the effect of foreign DNA introduction on these factors. Plasmids pEThIL-2 and pETmIL-4 were used for expression of human interleukin-2 [hIL-2] and mouse interleukin-4 [mIL-4] inside the cytoplasm of the cells. Having confirmed protein expression using SDS-PAGE analysis and ELISA assay, H2O2 concentration and catalase activity were measured at various ODs. Empty vector introduction increased significantly H2O2 concentration of the cells. However, H2O2 concentration in hIL-2 and mIL-4 expressing cells was significantly higher than its concentration in empty vector transformed cells. Catalase activity was reduced in foreign DNA introduced cells. It was more lowered following expression of recombinant proteins. Results of this study revealed the relationship between foreign DNA introduction and protein expression with H2O2 elevation and catalase activity reduction. There was also correlation between H2O2 elevation and reduction in catalase activity with the cell growth depression

[ study of complementary effects of cinnamon on hypoglycemia induced by insulin in male diabetized rats using sterptozotocin]

Background: traditional treatments for diabetes with natural plants and or its drivatives such as cinnamon are knowed throughout the world. The assessment of cinnamon bark on decreasing blood glucose treated by insulin in male streptozotocin-diabetized rats

Methods and materials: 40 male rats with average weight of 220 gr. were divided into four groups of 10: Control group, was taked only water and regular food. Diabetic group which is diabetized with 40 mg/kg intraperitoneal streptozotocin. Diabetic and insulin group, was treated by insulin NPH [5U/kg] after diabolized by streptozotocin. Diabetic and insulin and cinnamon group, was co-treated with insulin and oral cinnamon suspension. Plasma glucose was assayed using a glucose oxidize method

Results: in cinnamon group, the blood sugar was decreased significantly more than insulin group[p<0.01]

Conclusions: it is suggested, the bioactive compound[s] from cinnamon [probably MCHP-Methyl Hydroxy Chalcone Polymer] may potentiate the function of insulin in cinnamon group. However, the bioactive product[s] of cinnamon might provide a natural remedy against diabetes in future

Development of a mathematical model for myoelectric activity of small intestine to reach a better understanding of the pathophysiology of Irritable bowel syndrome [I.B.S.]

Since enteric nervous system is a complicated network and calls for more investigations, we decided to develop a model for myoelectric activity of small intestine by use of artificial nerve networks, in order to reach a better underst and ing of I.B.S. According to empirical knowledge concerning I.B.S, serotonin level in enteric nervous system increases and this system becomes more excitable and active. The aim of this study was to prove these issues by use of mathematical models. MTLAB 6.5 soft ware was used in this project. Artificial nerve networks, equivalent to enteric nervous system were designed. By making use of artificial nerve networks, amplitude, time and number of spikes in the myoelectric curve of small intestine were determined which were compatible with empirical information. Using artificial nerve networks, the effect of increased level of serotonin as a pathophysiological factor, on bowel movement in IBS was established. This study can pave the way for designing other artificial networks to assess potential effects of drugs; or developing more effective methods for treatment of I.B.S.