[Evaluation of infectious hematopoietic necrosis, infectious pancreatic necrosis and viral hemorrhagic septicemia viruses in Iranian and imported rainbow trout eggs: a cross sectional study]

Infectious hematopoietic necrosis [IHN], viral hemorrhagic septicemia [VHS] and infectious pancreatic necrosis [IPN] as important viral diseases of salmonids, especially rainbow trout can be led to mass mortality among cultured fish. The aim of the present study was to show if IHN, IPN and VHS viruses can be detected in Iranian and imported rainbow trout eyed eggs and to compare their abundances among the hatcheries of 3 regions [Farsan, Koohrang and Lordegan] in Chaharmahal va Bakhtyari Province. In each area, three hatcheries were selected, 20 eyed eggs were randomly sampled from each farm. The samples were transferred into the sterile tubes and identified by reverse transcriptase -PCR. Meanwhile, physicochemical factors were recorded for each fish. While total infection rate in eggs was 23.3%, the level of infection for IPN, IHN and VHS was 12.5%, 10% and 0.83%, respectively. In this respect, maximum and minimum frequency were observed in Farsan [19.15%] and Koohrang [0.83%]. Comparison of the infected of eggs, based on their origin, showed that the infection rates in Iranian and imported eggs were 20% and 3.33%, respectively. The results revealed that rainbow trout eggs should be considered as major source for transmission of aquatic viruses. Hence, molecular identification of above mentioned viruses in rainbow trout eggs should be done

Parasites of some freshwater fish from Armand river, Chaharmahal va Bakhtyari province, Iran

The aim of this study was to detect the occurrence of parasites in fish in Armand River, Chaharmahal va Bakhtyari Province regarding the importance of native fish population in the river. The occurrence of parasites was investigated in 6 native fish [Capoeta capoeta, C. damascina, C. aculeta, Barbus barbulus, B. grypus and Glyptothorax silviae] collected from the current main channel of the river from autumn 2009 to summer 2010. 63.7% of the studied fishes were infected with 19 parasite species including Ichthyophthirius multifiliis, Myxobolus musayevi, Dactylogyrus lenkorani, D. gracilis, D. pulcher, D. chramuli, D. akaraicus, D. skrjabiensis, two species of Gyrodactylus, Paradiplozoon sp., Lamproglena compacta, Copepodid of Lernaea cyprinacea, Ergasilus sp., Allocreadium isoporum, Allocreadium pseudaspii, Kawia sp., Bothriocephalus gowkongensis and Rhabdochona denudata. The infection rate was significantly higher [P<0.05] in C.aculeata while the maximum parasite diversity was found in C.damascina. The infection rate was also significantly different in four seasons [P<0.05] but no significant differences were found among fishes with different weight and length. High prevalence of Ichthyophthirius multifiliis and Rhabdochona denudata may affect native fish population. Monogenean parasites Dactylogyrus akaraicus and D. skrjabiensis collected from B. barbulus and C. capoeta are reported for the first time in Iran. B. barbulus is also reported as a new host for aforementioned parasites