ABSTRACT
Background: in man the immunosuppressive drug Cyclosporine A [Cs A] has been used successfully in organ transplantation and in the treatment of autoimmune disorders and exerts toxic effect on various parenchymal organs. This study was performed with the aim of testing whether Cyclosporine plus vitamin A would be reversed the effect of cyclosporine on rat's testis
Methods and Materials: 40 male Wistar rats, 2 months old were divided into four groups [10 in each group]. Experimental groups [A] were injected daily 40 mg/kg body weight of Cyclosporine and olive oil subcutaneously and in group [B] rats were injected daily 40 mg/kg body weight of Cyclosporine and olive oil plus vitamin A. In group [C] rats were injected only olive oil and in group [D] rats were normal and without any injection. Rats were kept under optimal hygienic condition, temperature 25 degreeC, relative humidity 40 to 45% and light provided for a 12-h day/12-h night cycle for 2 weeks. They were given water and rodent pellets. After 2 weeks rats were killed and testis were fixed in formalin 10% and were stained and studied with light microscopy
Results: distances between seminiferous tubules were increased. Accumulation of necrotic materials of the cells in the lumen of seminiferous, oligospermia, displacement of seminiferous tubules and changing the situation of sertoli nuclei were seen. All of changes were not seen in group [B]
Conclusions: we can use vitamin A as a protective in young patient against Cs side effects