[Effect of the cholesteryl ester transfer protein I405V polymorphism on the lipid response to changes of dietary fatty acid composition]

Atherosclerosis results from a complex interaction between genetic and environmental factors. Free cholesterol efflux from peripheral tissues and transferring to the liver for excretion from bile which is known as reverse cholesterol transfer [RCT] plays a central role in protection against atherosclerosis. HDL and cholesteryl ester transfer protein [CETP] are the major participants of RCT. The aim of the present study was to investigate whether the cholesteryl ester transfer protein I405V polymorphism modifies the response to changes in the dietary ratio of polyunsaturated to saturated fat [P:S]. The population study included 85 healthy subjects with the different l405V genotypes [35 II, 36 IV, and 14 VV] who assigned to two consecutive 28 days experimental period. All subjects consumed a high polyunsaturated fatty acids [PUFA] diet with P:S of 1.2 for the first period and a high saturated fatty acids [SFA] diet with a P:S of 0.3 for the next 28 days period. At the first and the end of each dietary period, serum lipid and lipoprotein concentrations were measured. At beginning of screening, lipid or lipoprotein concentrations were not significantly different among CETP I405V genotype groups. After the high SFA diet, subjects carrying V allele had a greater reduction in apolipoprotein A-I [apoA-I] and HDL cholesterol [HDL-C] than subjects with II genotype. The CETP I405V polymorphism contributes to the unfavorable changes of apoA- I and HDL-C when a high PUFA diet were replaced with a high SFA diet

[Study of correlation of surface phospholipids fatty acid composition in high density lipoprotein with the severity of coronary artery disease after angiography]

Background and Objective: the phospholipids fatty acid content of high density lipoprotein [HDL] has recently been found to be related to several important biological functions which may serve as a risk factor for coronary artery disease [CAD]. The purpose of this study was to investigate whether fatty acid composition of HDL phospholipids correlates with the presence and severity of coronary artery disease

Materials and Methods: the study population included 117 patients with coronary artery disease which was approved by angiography. The severity of CAD was assessed by the number of arteries [1, 2 or 3] with more than 50% stenosis reported by angiography. The fatty acid composition of HDL phospholipids was determined by gas liquid chromatography. The independence of association between fatty acids and CAD were evaluated by multivariate analyses which included all of the variables associated with the severity of CAD in univariate analysis

Results: these analyses showed that the association of EPA [beta= 0.23, P<0.01] and DHA [beta= 0.17, P<0.05] with the severity of CAD was inversely and independently significant

Conclusion: some kinds of polyunsaturated HDL fatty acid contents such as EPA and DHA [omega-3 fatty acids] have adverse association with severity of CAD