Respiratory epithelial cell lines exposed to anoxia produced inflammatory mediator / 대한해부학회지

Human epithelial cell lines were utilized to examine the effects of anoxia on cellular growth and metabolism. Three normal human epithelial cells lines (A549, NHBE, and BEAS-2B) as well as a cystic fibrosis cell line (IB3-1) and its mutation corrected cell line (C38) were grown in the presence and absence of oxygen for varying periods of time. Interleukin-8 (IL-8) levels were measured by enzyme-linked immunosorbent assay technique. Cellular metabolism and proliferation were assayed by determining mitochondrial oxidative burst activity by tetrazolium compound reduction. The viability of cells was indirectly measured by lactate dehydrogenase release. A549, NHBE, and BEAS-2B cells cultured in the absence of oxygen showed a progressive decrease in metabolic activity and cell proliferation after one to three days. There was a concomitant increase in IL-8 production. Cell lines from cystic fibrosis (CF) patients did not show a similar detrimental effect of anoxia. However, the IL-8 level was significantly increased only in IB3-1 cells exposed to anoxia after two days. Anoxia appears to affect certain airway epithelial cell lines uniquely with decreased cellular proliferation and a concomitant increased production of a cytokine with neutrophilic chemotactic activity. The increased ability of the CF cell line to respond to anoxia with increased secretion of inflammatory cytokines may contribute to the inflammatory damage seen in CF bronchial airway. This study indicates the need to use different cell lines in in vitro studies investigating the role of epithelial cells in airway inflammation and the effects of environmental influences.

[Determination of incidence rate of acute lymphoblastic leukemia in children under the age of 15 in Fars province in 2001]

Introduction and Objective: cancers in children involve a group of malignancies which are known to be the main cause of most common mortality in industrialized nations. The most serious malignant cancer in children is leukemia with an incidence rate of 30-33 per one million in American and European countries. This study was done to determine the incidence rate of cancer in 2001

Materials and Methods: the data were collected through face to face interviews with the patients' mothers and the questionnaires were filled out by the investigator. The data were analyzed by SPSS software

Results: according to findings of this study, the incidence rate of leukemia in Fars province was 32.6 [37.1 in males and 27.1 in females] per one million people under 15. Maximum of incidence was in 0-4 years age group and it happened more in urban than rural areas. The disease occurred more in boys than in girls [p<0.05]

Conclusion: on the whole, the incidence pattern of cancer in Fars is similar to those in European and American countries. Sex, age and place of residence appear to be important factors in the development of this disease