[Effect of valproic acid on Expression of Bim gene and viability of ovarian cancer cell line A2780]

Background and Objective: Ovarian cancer is the fifth common cancer among women and the number of new cases is increasing. Valproic acid is a histone deacetylase inhibitor effectively used to treat epilepsy and bipolar disease. Recently, this compound has attracted attention as an anti-cancer agent. Bim is one of the most important genes of mitochondrial pathway of apoptosis, and it plays an important role in the biology of cancer. Expression of this gene is greatly reduced in ovarian cancer. This study was done to evaluate the effect of valproic acid on the viability of ovarian cancer cells, apoptosis and Bim gene expression in A2780 line

Methods: In this experimental study, the human ovarian cancer cells [A2780] were grown in RPMI-1640 medium in appropriate culture conditions. The cells were treated by various concentrations valproic acid [1-30 mM] and were incubated for 24, 48 and 72 hours. After the incubation of period, cell viability was investigated using MTT. Apoptosis was analyzed by flow-cytometry method in the cells were treated by valproic acid. The Real time PCR test was used to assess the effect of this drug on the expression of Bim gene

Results: The results of MTT assay showed that valproic acid reduced the viability of A2780 cells, and this effect was time and dose-dependent. The reduction of cell viability at 30 mM concentration and 72 hours after treatment, was maximum and statistically significant [P<0.05]. Exposure to valproic acid significantly increased the percentage of apoptotic cells [P<0.05]. Also, Valproic acid significantly increased the expression of Bim [P<0.05]

Conclusion: Valproic acid reduced viability in ovarian cancer cell line A2780. Valproic acid increased cell death by altering the expression of genes involved in apoptosis in ovarian cancer cell lineA2780

Study of cloned gene-related protein Vp2 of infectious bursal disease virus-induced apoptosis in human lymphoma B cells in vitro

Apoptosis or programmed cell death [PCD] is an important mechanism in both development and homeostasis in adult tissue for the removal of superfluous cells, while its induction is an effective therapeutic approach for cancer. The aim of the present study was to evaluate the effect of cloned gene-related protein Vp2 of infectious bursal disease virus in human lymphoma B cells. In present study after cloning the Vp2 gene in Pichia pastoris system, the Vp2 protein was expressed. Cellular vital capacity was determined by MTT. Then effect of Vp2 protein in human lymphoma B cells was examined by Hoechst staining and flowcytometry techniques, respectively. During MTT, human lymphoma B cell lines revealed to have a meaningful apoptosis at 1 micro g and 5micro g protein concentrations when compared with controls [p<0.01]. Apoptotic bodies appeared by Hoechst staining apoptosis was induced suitably after 48 hours by flowcytometry assay. The present study is the first study that has revealed the gene-related protein Vp2 induced apoptosis in human lymphoma B cells in vitro