ABSTRACT
Background: Medicinal plants of Genus Ferula are prennial herbs of Apiaceae family which are widely distributed all around the word. Many of Feula species are exclusive and endemic to Iran which has widespread uses in traditional medicine as food additive, as well as a carminative, antispasmodic and expectorants. Coumarins, flavonoids and sulfur-containing compounds with different biological effects have been reported from the plant
Objective: The phytochemical investigation on total extract of the aerial parts of Ferula persica Willd var. Latisecta collected from the Central Alborz Protected Area was subjected with the aim of purification and determination of main metabolite constituents of the plant extract
Methods: Dried and powdered plant material was extracted by maceration method. Then the extract was concentrated under reduced pressure to leave a residue which was defatted and chromatographed on a silica gel column by increasing slightly the polarity with solvent. After the serial fractionation the fractions were compared by TLC, and those giving similar coumarin spots were combined and further purified on PLC to give a pure natural compound
Results: After the purification step the remarkable constituents were obtained. Elucidation of results by spectral methods [IR, 1H-NMR, 13C-NMR and DEPT] demonstrate that a coumarin namely: Auraptene [7-genaryloxycoumarin] was isolated from the aerial parts of F. persica. var. latisecta
Conclusion: F. persica is an endemic and endangered species of medicinal plants of Iran which contains different natural compounds such as coumarins. So the Protection of the plant for its economic and scientific uses is so important
ABSTRACT
Considering the and importance of formation of atrial Thrombus [AT] in patients who had developed atrial fibrilation [AF] and its early diagnosis, and with some regards to several reports on benefits of applying the D-Dimer test in such conditions, this study was carried out to detemine the accuracy and value of this test compared to the gold standard method of Trans-Esophageal Echocardiography [TEE] in patients referring to Modarres hospital. This clinical trial was performed on 73 patients whom were diagnosed as having AF. A more accurate method of TEE was applied to confirm if they had atrial thrombi. For determination of value of D-Dimer test in diagnosis of AT, 5 [cc] of blood was taken from any subjects. The results were regarded normal if the D-Dimer level was less than 500 ng/ml. Then positive and negative predictive values [PPV and NPV] were calculated as diagnostic measurments. Of the 73 subjects of this study [67% women versus 33% men] with mean age of 44.7 +/- 12 years old, there was 53.4% who had the history of Warfarin usage. According to the results of D-Dimer test, 33% had reached to positive levels, whereas 67% revealed to have no thrombus. The PPV of this test was calculated 48.6% and the NPV was 81.6%. Regarding the present condition of our patients and the excessive use of Warfarin among them and also probable failures of D-Dimer test, this test does not have the acceptible capability [particularly duringin these situations] in diagnosing AT