ABSTRACT
Background and Purpose: Thyroid autoimmune disease is the most common thyroid disorder in low-iodine intake areas; evaluation of thyroid peroxidase antibodies [TPOAb], anti- Thyoglubolin [TGAb] and anti-microsomal [TMAb] in most patients with hypothyroidism is of significant importance
Therefore, the present study was conducted to determine the role of anti-thyroid antibodies in patients with hypothyroidism in Sabzevar, Iran
Methods and Materials: This is a cross-sectional descriptive analytical study where 382 subjects with TSH above normal and T3 and T4 below normal were selected through non-probability convenient sampling. Their TSH, TPOAb, TGAb and TMSb were measured through Eliza method. The obtained data were analyzed in SPSS 15 using chi-square, independent t-test and Pearson correlation coefficient, where P< 0.05 was considered significant
Results: TPOAb, TGAb and TMSb in men and women with TSH higher than 10 mIU/ml were 72.3%, 78.3% and 50% positive respectively. In 72.3% of the subjects, TPOAb was above normal; in 78.5% of them TMAb was higher, and in 50% of them TGAb was higher. The highest rate of correlation was observed between TGAb and TPOAb [r=0.89]
Conclusion: The results indicated that, in the population studied, TPOAb, TMAb and TGAb were higher than the normal rates, and that TPOAb and TGAb are more remarkably changing to higher rates than TMAb
ABSTRACT
Background and Purpose: Keratinolytic fungi [KF] are among the fungi existing in the soil, whose enzymatic activity causes the decomposition of keratinized material in the soil; they are also considered as one of the pathogenic factors. The present study was conducted to determine the enzymatic activity of the KF, and to evaluate the effect of climatic conditions on their activity
Methods and Materials: This descriptive cross-sectional study was conducted on 90 isolates of KF randomly selected from different regions in Iran and other countries in 2004. The samples were cultured in Modified Czaspex-dextrose liquid medium [MCDLM]. Their enzymatic activity was measured using Azocazein, the general subtilisin of serin proteinase, and chromogenic substrates including N-Suc-Ala-Ala-Pro-Phe-NA, N-Suc-Ala-Ala-Pro-Leu-NA and N-Bz- Phe-Val-Arg- NA. The obtained data were summarized using two-dimensional tables, and were analyzed using non-repeating one-way ANOVA and Tukey test in SPSS
Results: The results indicated that Myriodontium keratinophilum [94.6 micro/ml] and Microsporum coockei [81.66 micro/ml] had the highest Azocaseinolytic activity, and hydrolysed N-Suc-Ala-Ala- Pro-Phe-NA, N-Bz- Phe-Val-Arg- NA and N-Suc-Ala-Ala-Pro-Leu-_NA respectively. Statistically significant differences were onserved to exist between methods of determining enzymatic activity [P<0.05]
Conclusion: The results indicated that the enzymes secreted in these fungi were of a proteinase nature and belong to the subtilisin-like serin proteinase
ABSTRACT
Assessment of genetic changes from a molecular viewpoint is essential for diagnosis, follow-up and treatment. For molecular procedures, DNA extraction is the first step. Therefore, applying economical and easy procedures will be helpful. Pathological specimens are normally fixed in formalin and paraffin after surgery and it is important to extract quality DNA from specimens. This study is intended to compare Chelex and Phenol / Chloroform methods of DNA extraction in Paraffin-embedded Colorectal Cancer specimens and their effect on PCR-SPSS. This descriptive-analytical study was conducted on 60 colorectal cancer specimens including 10 rectal and 50 various colon specimens. DNA extractions were done by Chelex and Phelex / Chloroform methods and their quality and quantity were measured by spectrophotometry. The P53 gene was amplified using specific primers in a PCR assay and, consequently, electrophoresis was done. The two methods were compared and analyzed by Kappa coefficient. Out of 60 subjects, 15 were female and 45 male. Their age ranged from 44 to 91 with the mean being 62 years. Quality DNA was extracted from 44 formalin-fixed specimens by Chelex method, and the PCR was positive. However, with the same sample, 32 specimens had suitable PCR assay by Phenol / Chloroform method. Therefore, a significant relationship was observed between the two methods [p<0.01]. The findings revealed that DNA extraction of paraffin-embedded specimens was better conducted by Chelex method rather than Phenol / Chloroform