ABSTRACT
Background and Purpose: Much evidence indicates that Mentha-piperita [MP] is traditionally applied for the treatment of gastrointestinal disorders, and probably reduces smooth muscle contraction and bowel movement. The present study was conducted to determine the effect of MP Hydroalcoholic extract on ileum movement in guinea pig ileum in invitro model
Methods and Materials: In this experimental study, we used 5 male guinea pigs [200-300gr]. After killing the guinea pigs, at least 6 pieces of ileum 2-3 cm long were dissected, and established in normal Tyrod solution plus carbogen gas in 37oC. Then, each tissue piece was fixed between two electrodes connected to stimulator apparatus. The contraction of ileum was carried to a physiograph by an isotonic transducer, and contractions were recorded. Then solutions with 5 concentrations of MP extract were added to the organ bath during ileum stimulation with stimulator. Then the physiograph recorded the contractions again. At least 6-7 responses were recorded
Result: Findings revealed that addition of Hydroalcoholic extract of MP in concentrations of 5, 15, 50, 150, 500 mg/ml during 0.1 Hz stimulation decreased contraction duration in a dose-dependent manner. The percent of these changes were 27%, 30.8%, 41%, 61%, and 75% respectively. Activity of 50% of this effect of MP was found to be in the dose of 50 mg/ml [P<0.05]
Conclusion: The results indicated that MP extracts can decrease ileum contraction and bowel movement in a dose-dependent manner