ABSTRACT
In the present study, antimicrobial activities of nine extracts from three medicinal plants indigenous to Sudan and commonly used in traditional medicine, namely Terminalia brownie, Acacia seyal and Tephrosia opollinea, were tested against four standard bacterial strains: Bacillus subtilis, Staphylococcus aureus, Eseherichia coli and Pseudomonas aeruginosa, and three fungal strains: Aspergillus niger, Aspergillus flavus and Candida albicans. The results exhibited that the chloroformic and methanolic extracts had prominent activity against most of the tested microorganisms. On the other hand, the benzene extract was devoid of any antimicrobial activity against all tested organisms. Due to the prominent antimicrobial activity of Terminalia brownie, it was subjected to an in-depth phytochernical investigation. Column chromatography of the methanolic extract resulted in the isolation of a crystalline compound [Tb/1]. The chemical structure Tb/1 was elucidated by infra-red coupled with nuclear magnetic resonance [1HNMR and 13CNMR] and electron impact mass spectrophotometer [ElMS] as beta-Lupeol. This compound was not previously reported from T. brownii. It exhibited a high antifungal activity against C. albicans