Immunohistochemical analysis of P53 protein in odontogenic cysts

The p53 is a well-known tumor suppressor gene, the mutations of which are closely related to the decreased differentiation of cells. Findings of studies on immunohistochemical P53 expression in odontogenic cysts are controversial. The present study was carried-out to investigate the immunohistochemical expression of P53 protein in odontogenic cysts. Thirty paraffin blocks of diagnosed odontogenic cysts were processed to determine the immunohistochemical expression of P53 protein. Nine of the 11 odontogenic keratocysts [81.8%] expressed P53, one of three dentigerous cyst cases expressed P53, while none of the 16 radicular cysts expressed P53 protein. The findings of the present work supported the reclassification of OKC as keratocystic odontogenic tumor

study of HER-2/neu expression and silver binding nucleolar organizer regions [Ag NORs] in perilesional normal salivary gland tissue

Salivary gland neoplasms comprise phenotypically and biologically diverse lesions of uncertain histogenesis. The present study was carried out to assess the immunohistochemical expression of HER-2/neu protein and of silver binding nucleolar organizer regions in normal salivary tissue. Twenty paraffin blocks of salivary tumors containing surrounding normal salivary tissue were used in the study. One section was processed to investigate HER-2/neu protein expression and another section was stained to assess the mean Ag NOR counts in normal salivary tissue. Only two cases of the studied normal salivary tissue showed HER-2/neu expression [10%], which was expressed only in ductal cells with 4.2 +/- 2.1 mean number of reactive cells per five high power fields [HPFs]. Statistically significant [P=0.01] higher mean Ag NOR counts in ductal [2 +/- 0.83] than in acinar cells [1.2 +/- 0.01] of normal salivary tissue were found. It was concluded from the data of the present study that ductal cells of normal salivary tissue are a proliferative pool and an oncogenic target which plays an important role in the histogenesis of salivary gland tumors

Urodynamic evaluation after Stamey's bladder neck suspension

Between January 1993 and December 1995, eighteen patients were subjected to Stamey's bladder neck suspension at our center, with an overall success rate of 94.4%. All patients had preoperative uroflowmetry, water cystometry and profilometry using water-filled catheters. Resting and stress urethral pressure profiles were recorded at 3, 6, 9 and 12 months postoperatively and then every 6 months thereafter. The mean follow-up was 1 year. Patients in whom surgery was successful were further subdivided into two subgroups [A and B] for the purpose of comparison. Group A included 12 patients who had achieved complete cure and group B 6 patients who showed improvement. Success was assessed both subjectively [through direct questions given on subsequent follow-up visits] and objectively [through doing a stress test at each visit]. It was found that different profilometry variables showed a statistically significant increase in postoperative traces for the successful group in general, and a significant difference existed when comparing the two subgroups

Bstructive anuria, [management appraesal]

Post renal [obstructive] anuria is a common urologic emergency in Egypt. In this study, a retrospective analysis of 170 patients admitted with obstructive anuria is presented. The most common cause of obstrcution was stones [62.3%] and ureteral strictures presented 24.7% of the cause. Only 13% of the patients presented with bilateral obstruction and the remaining had single functioning obstructed kidneys. Most of the patients were drained with PCN with success rate of 90%. After drainage and correction of the uremic, half of the patients had corrective surgery for the obstructing factore, one fourth had spontaneous diuresis with of small stones and the remaining fourth had permenant nephrostomy for drainage in view of the poor renal function or the poor cardio-pulmonary status. Our policy is to use primarily the PCN for draingae ast it was observed that it is a simple; procedure that increases the overall cure rate and reduces the overall mobidity and mortality

9 year's experience with management of branching stones

102 patients having staghorn stones were managed, between 1975, 1984. Patients were evaluated medically, radiologically, bacteriologically and their renal function was assessed by creatinine clearance and in some cases by renogram. Different lines of management were utilized. Extended pyelolithotomy in 30 cases, Extended pyelotomy and multiple nephrotomies in 3, anatrophic nephrotomy in 23, lower polar partial nephrectomy in 15, nephrectomy in 17 and medical treatment in 19 patients. [5 patients underwent two operations]. 75 patients could be followed up using the same preoperative investigation with the addition of typing of the incecting organism. Residual stones were within the reported figures 29.5%. Infection was controlled after surgery from 80% but it increased with medical treatment. Morbidity was minimal and no mortality was reported among cases treaed surgically

Adenocarcinoma in bilharzial bladder: a retrospective study of 41 cases

Our experience with 41 patients with vesical adenocarcinoma treated between 1969 and 1983, is presented. The operative mortality was 9.5%. The 5-year actuarial survival of cases treated by radical surgery was 38.9%. The relation between treatment failures, histologic types of adenocarcinoma [Non mucin, mucin producing and mucinous] and grade is outlined

Visual internal urethrotomy for stricture urethra

50 patients with stricture urethra were treated with V.I.U. in our department and follow up after 6 months. The bulbous urethra was the commonest site of structure in 17 patients [34%]. The most common cause of stricture was traumatic [36/50]. The complications were minor and only in 14%. The success rate was 88%. Urethral dilatation is essential in most of the cases to maintain urethral patency