Induction toxoplasma gondii in goats

The prevalence of anti-Toxoplasma gondii antibodies was investigated in goats slaughtered for food in various areas of the Giza province. Egypt. Two hundred and thirty blood samples collected from the slaughtered goats were used for the serologic diagnosis of T. goadii infection by the modified agglutination test [MAT] with cut-off value 1:25. The antibodies to T. gondii good/i were found in 44.3% [102/230] of goat sera. Experimental infection of goats with the infective stages of both locally isolated and RH strains of T. gondii was carried out. Persistence of T. gondii tissue cysts and lesions were demonstrated in the different tissues and organs of the experimentally infected goats by the histo-pathological examination, This study is the 1st assay of toxoplasmosis in Egyptian goats by MAT and is the unique successful determination of the T. gondii infective stages within the experimentally infected goat tissues by the histo-pathological examination. Consequently the finding results obtained scope the public health significance of goat meat as source of human infection

Water quality and parasites biodiversity in treated wastewater

The present study aims to evaluate the performance of Helwan wastewater treatment plant. Cairo, Egypt with parasites biodiversity. The study included water quality after treatment and the occurrence of parasites. The treated wastewater [tww] samples were monthly obtained as well as tap water was monthly examined for water quality parameters. Diagnosis of parasite eggs and larvae were also achieved. The results showed that, the plysico-chemical parameters value were markedly higher in tww compared to tap water. Entamoeba histolytica cysts and Ascaris eggs were detected during all the time of examination. Coccidia oocysts and nematode larvae were recorded during June to August. Fasciola encysted metacercariae were also detected and for the first time during September to March. At the same time tap water samples were free from such parasites

Health aspects of fish cultured in reused wastewater with special reference to parasitic infestation

The present study aims to evaluate the application of recycled treated wastewater [tww] on fish culturing with special reference to productivity and health aspects. Samples of treated wastewater were monthly obtained, from Helwan wastewater treatment plant. Cairo, Egypt. The collected water samples were subjected to be used in laboratory fish culture. Growth and productivity of cultured fingerlings Oreochromis niloticus, reared in tww compared with that reared in dechlorinated tap water were recorded. Statistical analysis revealed no significant monthly changes in body weight, length and width between fingerlings cultured in tap water and tww Parasitological examination of fingerlings reared in tww revealed that only the skin and gills were affected by ptotozoan infection. At the same time fingerlings cultured in tap water were found free from parasites

Redescription of some monogenean parasites belonging to family: Ancyrocephalidae, bychowsky, 1937

Gills of 782 fish samples collected from the Suez Gulf Red Sea were examined for monogenean parasites. Four species of monogenean parasites belonging to family; Ancyrophalidae, were recorded. Pseudohaliotrematoids polymorphus eilaticus, and Pseudohaliotrema plectocirra from siganus reviulatus, Ancyrophalus atherinae, from Letherinus elongates and L. variegates and Haliotrema austral, from sclopsis ghanum

Structural characterization and immunolocalization of egg antigens cross react with Toxicara vitulorum, Fasciola gigantica and moniezia expansa mature flukes

A structural homology between eggs of Toxocara vitulorum, Fasciola gigantica and Moniezia expansa was proved by the use of SDS-PAGE. In immunoblot, nine, eleven and seven polypeptides were recognized in F. gigantica, M. expansa and T. vitulorum eggs, respectively, by their respective rabbit anti-adult antisera. Moreover, components of 240 KD and 206 KD were recognized in the three eggs by different anti-adult antisera. The anatomic localization of the cross-reactive epitopes in eggs was determined by indirect immunofluorescence microscopy. The cross- reactive epitopes were mainly associated with embryonated cells of F. gigantica, egg shell, larvae and vitelline membranes of T. vitulorum and egg shell and granular layer of M. expansa

Effect of bacillus thuringiensis on the immature stage of ornithodors savingnyii [argasidae]

The potential activity for the two varieties of Bacillus thuringiensis [kurstaki and israelesis] was evaluated against fourth instar nymph of Ornithodoros savignyli. Bacillus thuringiensis [Dipel-2x] was more effective than B. thuringiensis israelensis [Vectobac]. The effect of this bacterium on the haemolymph protein alteration of previously mentioned tick using SDS-PAGE electrophoresis, and the histopathological changes of the nymphs were also studied

Nematicidal effect of bacillus thuringiensis on trichostrongylus colubriformis [nematode]

The lethal effect of Bacillus thuringiensis [B.T.] var kurstaki and its toxin on eggs and larvae of Trichostrongylus colubriformis was studied. The ovicidal and larvicidal activities were higher with prolonged exposure of parasitic eggs and larvae to the bacteria and its toxin. The ovicidal activities of the bacteria and toxin were increased to be 93.71% and 93.66% respectively at 2.5 mg concentration. The larvicidal effects of bacteria reached lo 100% and 98% for toxin at 2 mg. Also the anthelmintic activity of B.T. kurstaki against T. colubriformis in rabbit was proved to be helpful

Field application of bacillus thuringiensis var kurstaki [dipel-2x] against soft and hard ticks

Field application of Bacillus thuringiensis [B.t.] var. kurstaki [Dipel-2X] against hard tick, Boophilus annulatus and soft tick, Argas [percicargas] persicus was probed. Hard tick died on infected calves after 6 days post-spraying with Dipel-2X, while soft tick succumbed in its cracks after 6 weeks post treatment. The indirect effect of Dipel-2X on Argas [p.] persicus was also investigated. Egg laying of tick fed on hens injected with 20 and 40 mg Dipel-2X was clearly affected. Molecular structures of hemolymphs collected from ticks got Dipel-2X infected blood meals did not change as demonstrated by SDS-PAGE

Theileriosis in cattle and buffalo of the new valley governorate, Egypt

A survey of cattle and buffalo showed that Theileria sp. was the only blood parasite detected in new valley and the percentage of infection was, respectively, 59.36% and 23% also other clinical symptoms, in addition to P.M. lesions, occurred in infected calves

Isolation and characterization of the most immunogenic fraction of Trichostrongylus colubriformis

Six fractions [F[1]-F[6]] were observed from Trichostrongylus colubriformis phosphate buffer extract separated by column chromatography using Sephadex G 200. Only three fractions [F[1], F[2] and F[3]] were antigenically specific in precipitating and haemagglutinating antibody responses

Cross transmission of trichostrongylus colubriformis between sheep and rabbits

Rabbits [Lepus Caniculus] were experimentally infected with cultures of mixed infective stage of Trichostrongylus colubriformis, T. axei, ostertagia spp. And Nematodirus spp of sheep Origin. Rabbits were only infected with T. colubriformis. A pure culture of T. colubriformis were made to infect lambs experimentally. The pre-patent period and number of eeg per grams were determined in every experiment

Health risks associated with parasitic agents in municipal sludge

Land application of the sludge may transmit different parasitic organisms. helminth eggs, larva and protozoan cysts were detected during, examination of the sludge. They were eggs of Ascaris lumbricoides Trichuris spp, Echinochasmus perfoliatus larvae of Ancylostomat sp Trichostongylus axei, T. colubriformis strongyloides spp, cysts of Entamoeba histolytica and cocysts of Eimeria spp