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1.
Bulletin of the National Research Centre. 2003; 28 (4): 431-51
in English | IMEMR | ID: emr-121082

ABSTRACT

The effect of exogenous ABA treatment on eight maize inbred lines [Zea maysL.] differing in their salt tolerance, with regard to some yield-related traits was examined. Also, fingerprints for the two salt-tolerant and the two salt-sensitive inbreds using RAPD-PCR markers were identified. From 20 maize inbred lines, four were chosen as salt-tolerant genotypes [L123a, G251b, L113a and Rg25] and four as salt-sensitive genotypes [Rg5, G227b, Rg1 and G13]. These eight maize inbreds were treated with 10-5 M abscisic acid [ABA] under5000 and 7000 ppm NaCl conditions. The effect of exogenous ABA on salt tolerance was tested by estimating some yield-related traits [plant height, total root length, shoot fresh weight, shoot dry weight and root dry weight]. The two most salt-tolerant [L123a and G251b] inbreds and the two most salt-sensitive [Rg1 and G13] were selected for detecting some biochemical and molecular genetic markers associated with salt tolerance. SDS-PAGE banding pattern showed differential responses with respect to salt tolerance and ABA treatment, while esterase and peroxidase isozyme profiles were weakly or negatively correlated with salt tolerance and ABA treatment. RAPD-PCR analysis showed that four random 10-mer primers, out of six, succeeded in differentiating salt-tolerant [L123a and G251b] from salt-sensitive [Rg1 andG13] inbreds, some specific markers for maize inbreds across RAPD-PCR analysis were determined. Twenty-seven of 57 RAPD markers were found to be inbred-specific, phylogenetic relationships between these inbreds were studied on the bases of RAPD-PCR patterns. Dendrogram analysis showed that, the inbreds were divided into two main groups and the most dissimilar one was the most salt-sensitive


Subject(s)
Zea mays , Salts , Electrophoresis , Peroxidases , Polymerase Chain Reaction
2.
Journal of the Egyptian Society of Parasitology. 1992; 22 (1): 1-7
in English | IMEMR | ID: emr-24192

ABSTRACT

Of 5,404 domestic dogs examined for normal and abnormal Taenia pisiformis in Cairo, Egypt, one dog contained 3 triradiate specimens while 3,569 harboured one or more normal cestodes. The study revealed a ratio of one triradiate to 5,620 normal adult worms in the parasite population and a prevalence of 0.02% for the triradiate forms. Polyradiate forms are far less common in this large survey than has been indicated in previous reports


Subject(s)
Animals , Microscopy, Electron, Scanning
3.
Journal of the Egyptian Society of Parasitology. 1991; 21 (3): 597-610
in English | IMEMR | ID: emr-20388

ABSTRACT

The epidemiology of Taenia pisiformis infections in domestic dogs in Cairo, Egypt. Over 5000 street dogs humanely killed in a culling programme organized by the Egyptian Ministry of the Interior in the winter of 1986 and the spring of 1987 have been utilized to examine the final host epidemiology of the cestode Taenia pisiformis in Cairo. In the winter months the prevalence of infection was 63.4% and the mean intensity 2.82 per dog: by the spring of 1987 these values had risen to 70.8% and 3.78 respectively. These parasite burdens probably represent the highest infection levels with T. pisiformis yet described from urban dog populations anywhere in the world. The dispersion of T. pisiformis within the street dog population was assessed and found to be significantly aggregated and well described by a negative binomial model of overdispersion [variance/mean ratio : 5.16, coefficient k = 0.795]. A separate analysis was carried out of infection in each of 18 circumscribed regional areas of Cairo which revealed considerable area-to-area differences with mean intensities varying between 1.62 and 6.54 and prevalences between 43.3% and 91.2%. The size structure of the winter and spring worm samples were very similar with about. 45% of worms less than 35 cm in length, about 35% between 35 and 59 cm, about 20% between 60 and 100 cm and less than 5% greater than 100 cm in length. The epidemiological factors which lead to high T. pisiformis infection levels in Cairo dogs have been considered and the consumption of discarded rabbit offal containing Cysticercus pisiformis by street dogs is regarded as a crucial mode of transmission


Subject(s)
Animals , Dogs
4.
Acta Medica Iranica. 1982; 24 (1-2): 17-35
in English | IMEMR | ID: emr-1426

ABSTRACT

In a retrospective study on the cancer of the gall bladder, done in Iran, it seems that it comprises 0.36% of all the malignant tumors. 1.75% of the patients were females and the maximum incidence was in the sixth decade of life. It was interesting to note that the age ratio between the males and females reverses with the advance of age, so at the 7th and 8th decade of life, the incidence of the disease in males was much higher than females. At the 8th decade, the number of male patients was twice as females. The presenting symptoms in most cases were; pain, jaundice, loss of weight, and in a few cases there was a history of long lasting symptoms, referable to the biliary system. After admission and surgical opening of the abdomen, 60% of patients were considered inoperable. All the patients suffering from anaplastic carcinoma, which comprised 5% of all cases, were female and had a long lasting history of gall bladder infections. The prevalence of stone formation in gall bladder malignancies is a known fact but whether the cause of this coincidence is due to some factors, such as irritation dysplasia,or only comibination of these, is not definitly known


Subject(s)
Retrospective Studies
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