Effect of abscisic acid on some salt stressed Maize Inbreds

The effect of exogenous ABA treatment on eight maize inbred lines [Zea maysL.] differing in their salt tolerance, with regard to some yield-related traits was examined. Also, fingerprints for the two salt-tolerant and the two salt-sensitive inbreds using RAPD-PCR markers were identified. From 20 maize inbred lines, four were chosen as salt-tolerant genotypes [L123a, G251b, L113a and Rg25] and four as salt-sensitive genotypes [Rg5, G227b, Rg1 and G13]. These eight maize inbreds were treated with 10-5 M abscisic acid [ABA] under5000 and 7000 ppm NaCl conditions. The effect of exogenous ABA on salt tolerance was tested by estimating some yield-related traits [plant height, total root length, shoot fresh weight, shoot dry weight and root dry weight]. The two most salt-tolerant [L123a and G251b] inbreds and the two most salt-sensitive [Rg1 and G13] were selected for detecting some biochemical and molecular genetic markers associated with salt tolerance. SDS-PAGE banding pattern showed differential responses with respect to salt tolerance and ABA treatment, while esterase and peroxidase isozyme profiles were weakly or negatively correlated with salt tolerance and ABA treatment. RAPD-PCR analysis showed that four random 10-mer primers, out of six, succeeded in differentiating salt-tolerant [L123a and G251b] from salt-sensitive [Rg1 andG13] inbreds, some specific markers for maize inbreds across RAPD-PCR analysis were determined. Twenty-seven of 57 RAPD markers were found to be inbred-specific, phylogenetic relationships between these inbreds were studied on the bases of RAPD-PCR patterns. Dendrogram analysis showed that, the inbreds were divided into two main groups and the most dissimilar one was the most salt-sensitive

Study of diabetes mellitus among pulmonary tuberculosis patients in chest hospital in Ismailia and Suez cities.

Many early investigators recognized the increased risk of development of tuberculosis in diabetic. No careful inquiry was made into the incidence of diabetes in tuberculous populations. A title of 105 inpatients cases of pulmonary tuberculosis and 54 non tuberculous attending the outpatients clinic of chest hospital in Ismailia and Suez cities. Both groups were subjected to full clinical examination and oral glucose tolerance test, 22.86% of tuberculous patients were proved to be diabetic. 62.5% of tuberculous diabetics had bilateral lung affection, 45.8% had upper and mid zone affection. 41.6% had infiltrative lesions. 33.3% infiltration and cavity and 25% had cavitary lesion. Highest percent were found in age group 20 - 29, and in males [75%]. Diabetes mellitus is common among pulmonary tuberculosis patients. Efforts for early discovery of diabetes mellitus among tuberculous patients should be endeavored

Immunoglobulin levels in lung lavage in bilharzial patients with or without generalized airways obstruction

The relationship between immunoglobulin [Ig] levels in bronchial lavage [BL], broncho-alveolar lavage [BAL] fluids and serum was assessed in bilharzial patients with or without generalized airways obstruction, and in normal volunteers. In normal subjects, both IgG/albumin ratio, [39.4+3], and IgE/albunin ratio, [4.1+0.4] in peripheral blood were equivalent to those in BAL fluid, [40+7] and [4.04+0.53] respectively, suggesting that transudation of IgG and IgE frolll blood is partially responsible for the IgG and IgE content in BAL. However IgA/albumin ratio was higher in BL, [19.9+6] and in BAL [32+7] than in serum [7+1.9] which suggests that local secretion in the main source of IgA in BL and BAL fluids in normal subjects. Serum and BAL fluids from patients with schistosomiasis with or without generalized obstructive airways disease, [GOAD], had a marked increase in the levels of IgG/albumin and IgE/albumin when compared to normal controls. By contrast to normal subjects, in bilharzial patients, IgG level in BAL significantly higher when compared to serum levels suggesting their local production in the lung