Enhancement of protease productivity from streptomyes graminofaciens, an antinflammatory and wound healing enzyme

Improvement of the protease productivity of Streptomyces graminofaciens through optimization of the cultivation conditions. Streptomyces graminofaciens was isolated from soil sample collected from West Area of Saudi Arabia. It is characterized by high protcase productivity. The effect of different environmental factors on the protease productivity of this isolate was determined by gelatin clearance zone technique [GCZ]. The maximum production of protease enzymes by S. graininofaciens was obtained after 96h incubation period at 30 C incubation temperature using the basal medium supplemented with 0.2% glucose 2% peptone, and 0.2% iso-leucine, adjusted at pH 7 using 0.2M phosphate buffer. Optimization of the environmental condition for growth of Streptomyces graminofaciens increased the protease production 2.6 times more than that produced by the control medium

Investigation on chlamydiae in Nile fish [sarothrodon nilotica] and common carp, [Cyprinus carpio L]

Unsuccessful attempts for detection of Chlamydiae infection were performed on 600 impression smears from liver and spleen of tilapia and carp to demonstrate elementary bodies of Chlamydial infection

Microbiological monitoring of meat-cold stores

A total of 50 swab specimens were obtained from governmental cold stores, 10 from each of the walls, floor and roofs as well as air and water. All samples were examined microbiologically for detection of contamination with mould, yeast and psychrotrophic bacteria. The mould that have been isolated are: Cladosporium, Penicillium, Mucor, Aspergillus, Monilia, Trichoderma, Alternaria, Paecilomyces, Sporotrichum, Geotrichum c and idum, Rhizopus and Scopulariopsis, while the yeast genera that have been isolated are; C and ida torulopsis, Rhodotorula and Saccharomyces. On the other h and, the mean values of the Psychrotrophic bacteria detected from floor, wall, roof, air and water were 1.6 x 10[4], 1.8 x 10[3], 3.0 x 10[2], 3.7 x 10[2] and 1.8 x 10[4], respectively. The spoilage and public health significance of the recovered strains were discussed

Enterobacteriaceae of slaughtered carcasses of sheep and goats with special references to salmonellae

A total of 150 samples from muscles, livers, intestines, mesenteric and hepatic lymph nodes were collected from 30 carcasses of sheep and goats [15 of each], slaughtered in Damanhour abattoir and examined bacteriologically for detection of enteric organisms. Proteus spp. were the highest isolates from carcasses of sheep [41.714%], followed by E. coli [23.076%], Enterobacter cloaca [15.384%], Arizona and Shigella sonnei [7.692% of each] as well as Aeromonas spp. and Citrobacter freundii [2.567% of each]. Also, Proteus spp. were recovered from carcasses of goats in highest proportion as 50%, followed by Enterobacter aerogenes [22.5%], E. coli [12.5%], Providencia [5%], and Arizona, Citrobacter violaceum, Edwardsiella and Klebsiella aerogenes [2.5% of each]. Salmonellae failed to be isolated from examined samples. Spoilage effect as well as the public health significance of isolated species were discussed