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Egyptian Journal of Biophysics and Biomedical Engineering. 2004; 5 (2-3): 167-174
in English | IMEMR | ID: emr-65744

ABSTRACT

Tumor angiogenesis is an important step for tumor growth and metastasis. There are specific growth factors operating in this process such as VEGF. The aim of this study is to examine the effect of single exponential electric field pulse of different intensities from 0.3 kv/cm to 1.65 kv/cm and 1 ms duration on the VEGF level in the cultured human C.N.S tumor cells. VEGF level is determined by Enzyme Immunoassay [EIA] technique in cell culture and its supernatant. The results showed that high intensity electric field pulse decrease VEGF level in the cells. Also, it was found that the rate of growth of tumor cells decreased with field intensity elevation as indicated by morphological observations


Subject(s)
Humans , Tumor Cells, Cultured , Central Nervous System Neoplasms , Electroporation , Cell Membrane Permeability
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