[ Study of Complications in 1120 Cases of Transvaginal Ultrasound - guided Oocyte Pick-up in IVF Centre of Yazd]

To determine the prevalence of oocyte pick up complications in 1120 cycles of IVF. In this prospective study 1120 pationts that were reffered to infertility clinics for IVF were enrolled. The patients with the infertility following COH by regimens of CC + hMG/hCG, hMG/hCG, hMG + GnRH-a/hCG were punctured by transvaginal sonography, preformed after injection of sedatives. The follicular aspiration was performed only by vaginal preparation with sterile distilled water .The patients were discharged in an hour. Vaginal bleeding was observed in 190 cases [17%], who were not severe and treated with vaginal pack and discharged after 2 hours after exit the vaginal pack. 15 patients [1.3%] with severe abdominal pain were hospitalized for 24 hours, one with hypovolemic signs was operated by laparoscopy and ruptured ovary was cauterized. 3 cases [0.35%] with fever, chills and abdominal pain managed with diagnosis of PID. One patient with history of 2 previous laparatomy before IVF cycle, didn't respond to medical treatment and after further investigation pararectal abscess diagnosed which was managed surgically. Considering the number of patients, transvaginal follicular puncture is a safe procedure in oocyte retrieval.In patients with history of PID, previous surgery and endometrioma prophylactic antibiotic was given at the time of oocyte puncture

[Does intracytoplasmic sperm injection overcome the reduce of fertilization rate by increasing maternal age?]

The aim of this study was to determine the effects of maternal age in fertilization rate of intracytoplasmic sperm injection comparison with IVF. In this prospective clinical trial study, 200 couples with male subinfertility that referred to infertility clinic were enrolled. Couples were selected on the basis of semen parameters and divided randomly into two groups of IVF and ICSI. Couples were divided into different subgroups based on age of females .The fertilization rate of every subgroups were assessed and compared together. Results were analyzed by Chi-square method. After induction ovulation with standard protocols, 525 oocytes were retrieved during egg collection in ICSI groups versus 474 in IVF. After selection of good quality oocytes, IVF and ICSI was done as routine .The fertilization rate of every age group was measured and compared with age increased. In IVF group, the fertilization rate was 41.3% in patients <35 years versus 26.7% in older than 35 years old .The mean fertilization rate was 89.3% in patients <35 comparison with 75.2% in women >35 years old by ICSI .On the other hand, the mean cleavage rate of IVF cycles was significantly lower in patients >35 years in IVF [49.5%] versus younger patients [P value<0.001] but this finding was not observed by ICSI [85.17% VS 85.15%].Reduction of fertilization rate with increase of female age was statistically meaningful [P value <0.001] in IVF and ICSI. These findings suggest that fertilization of oocyte reduce consequently with maternal age increased, therefore, ICSI can not overcome this problem their cause is not clear and may refer to chromosomal or structural abnormality in oocytes