Listeria monocytogene isolated from frozen bull semen

A total of 96 imported frozen semen straws represent 11 lots [groups] from different localities at different times during the last four years, revealed the isolation of Listeria monocytogenes from 14 samples by direct and cold enrichment procedures. Eleven isolates which were CAMP positive proved to be pathogenic to mice. Nine isolates could be identified biochemically and serologically where 5 isolates were identified only biochemically. The bacteriological count ranged from 4500-9500 cells/straw and the highest count was in group 4

Listeriosis in an Egyptian dairy cow herd inseminated with infected frozen semen with Listeria monocytogenes

An Egyptian dairy farm [557 cows] located in lower Egypt in Sharkia governorate was subjected to clinical and laboratory investigation following a continuous complain, represented mainly in low fertility rate. This farm used infected frozen semen with L. monocytogenes in artificial insemination. Listeriosis was diagnosed in different cows showed the typical reproductive and septicaemic form of the disease, also pigeons bred in the farm showed the typical nervous signs of circling disease. L. monocytogenes was isolated from 3 out of 12 soil samples taken from different areas from the farm. In addition, L. monocytogenes was isolated from the intestinal contents of apparently normal cows. Fourty isolates from cows, pigeons and soil, along with imported frozen semen were serotyped and all of them belonged to serovar 4 b and all isolates were pathogenic for white mcie