H-NMR spectroscopy of haemoglobin molecule in different anaemic illness

1H- NMR spectroscopy is an excellent technique for investigating the structure-function relationship of Hb. Due to the presence of the unpaired electrons in the high spin ferrous atoms in deoxy Hb and owing to the presence of the highly conjugated porphyrins in the Hb molecules, the proton chemical shifts of various Hb derivatives cover a wide range. 1H-NMR researches of paramagnetic hemoproteins have shown that the heme proton spectra can be used to probe the heme environment in three of anaemia