ABSTRACT
Neural stem cells can difrentiate to mature neural cells. Neural stem cells can migrate and repair the damage neural tissue. This study was done to determine the effect of hydro-ethanolic extract of Chamaemelum nobile on cell prolifration and apoptosis of rat hipocample neural stem cells in the oxitative stress condition. In this experimental study, neural stem cells were isolated from hippocampus of neonatal rat brain. Isolated neural stem cells were treated at 200, 400, 600, 800 and 1000 microg/ml of hydro-ethanolic extract of Chamaemelum nobile for 48h. Cells proliferation rate were evaluated by MTT assay. Anti-apoptotic property of hydro-ethanolic extract of Chamaemelum nobile evaluated using TUNEL assay method. Proliferation of neural stem cells were significantly increased in Chamaemelum nobile extract group in comparision with control [P<0.05]. The rate of apoptotic cells were significantly reduced in Chamaemelum nobile extract group compared to control [P<0.05]. The hydrethanolic extract of Chamaemelum nobile increases proliferation rate and reduces apoptosis of neural stem cells in the oxitative stress condition