Histopathological changes in the stressed rat's liver following repeated exposure to halothane

Twenty four rats divided into four equal groups were used. Group I was kept as a control. Group II was exposed to halothane 2% for 20 minutes every other day for 13 exposure. The animals of Group III and IV were subjected for 20 minutes of stress by hitting on their cages in non-rhythmic fashion. Group III was exposed to oxygen. While Group IV was exposed to 2% halothane in oxygen for 13 exposures similar to Group II. Liver sections were stained with H and E., PAS and sundan blackstains. Degenerative changs in the hepatocytes appeared following exposure to halothane, while necrosis of the hepatocytes was seen following stress in Group III and became marked with exposure to halothane after the period of stress in Group IV. Glycogen content decreased particularly with halothane. Lipid content decreased with halothan exposure in Group II but relatively increased with stress in Group III and the hepatocytes became loaded with fat granules with halothane and stress in group IV

Hepatic histochemical changes of the stressed rats following repeated halothane exposure

Twenty rats, divided into 4 equal groups were used. Group I was kept as a control. Group II was exposed to halothane 2% for 20 minutes every other day for 13 exposure. The animals of Group III and IV were subjected for 20 minutes to stress by -hitting on their cages in non-rhythmic fashion. Group III was Exposed to oxygen, while Group IV was exposed to 2% halothane for 13 exposure similar to Group II. Liver sections were stained to detect enzyme activity. Alkaline phosphatase showed moderate increased activity in all groups and it was strong in Von Kupffer cells, cellular infiltrate and bile ducts. Succinic dehydrogenase enzyme was depressed in all groups, however, strong activity was seen in focal hepatocytes in Group IV, Von Kupffer cells and cellular infiltrate. Repeated halothane exposure depressed acid phosphatase activity in Group II but strong activity was seen in the middle and peripheral zones in Group III and IV

Histopathological and histochemical changes in rat's testes following repeated halothane exposure

Three groups of rats, 4 animals each, were used. Group I was kept as a control. Group II was exposed to halothane 1.5% for 20 minutes every other day for 13 exposures. Phenobarbtion 0.1% in milk was given to the rats Group III which were similarly exposed to halothane. Sections from the testes were stained with H and E, PAS and for detecting alkaline phosphatase activity. There were marked testicular atrophy, degeneration of the germinal epithelium and necrosis of the interstitial tissue in Group II. While more advanced changes were seen in Group III, seminiferous were atrophied, widely separated and contained no sperms. The intersititialaltissue was completely fibrosed. Alkaline phosphatase enzyme showed strong +ve activity in the thick walled blood vessels and the basement membrane of the seminiferous tubules in group II, and a weak +ve actifity in the degenerated testicular tissue in Group III

Histochemical changes in pregnant rat's liver following repeated halothane exposure [1]

Eighteen adult white rats were divided into 3 groups, 6 rats each, 2 males. The first group was exposed to 1.5% halothane for 20 minutes, every other day, for 6 exposures. The rats mated and exposed for another 10 exposures. The second group was given 0. 1% phenobarbitone in milk, one week before and during the period of exposure, which similar to the first group. The third group was kept as a control. At the suspected date of delivery, the rats weresacrified and specimens were taken from the mother's liver for estimation of the activities of the enzymes succinate dehydrogenase, acid phosphatase and alkaline phosphatase. Repeated halothane exposure decreased the activity of the enzymes succinate dehydrogenase, acid phosphatase and alkaline phosphatase. Treatment with phenobarbitone has the same effect on succinate dehydrogenase, but partially spared the activities of acid and alkaline phosphatases

Histopathological changes in pregnant rat's liver following repeated halothane exposure [2]

Adulte white rats were divided into 3 groups, 6 rats each [2 and 4]. The first group was exposed to 1.5% halothane for 20 minutes every other day for 6 exposures. The rats were mated and exposed another 10 exposures. The second group was given 0.1% phenobarbitone in milk one week before, and during the period of exposure which was similar to the first group. The third group was kept as a control. At the suspected date of delivery, the rats were sacrified, and specimens were taken from the liver for haematoxylin and eosin stain, PAS reaction and Sudan black stain. Repeated exposure to halothane did not affect the pregnancy rate, but it resulted in hepatic focal and centrilobular necrosis with glycogen poverty and moderate lipid content. The portal spaces showed thick connective tissue and lymphocytic infiltration. Phenobarbitone reduced the pregnancy rate and resulted in 16.5% foetal mortality with more hepatic necrosis and increases in glycogen and lipid content