Biotypmg of Pasieurella multocida isolates isolated from chickens in Egypt by RAPD PCR

The incidence of P. multocida isolates isolated from 50 random samples collected from chicken suffering from respiratory manifestation was 48%, the random serological classification showed 4 isolates [A:5, A:8, A:9and D:2]. RAPD-PCR using 4 random primers separately was demonstrated to characterize, identify and determine the differences in the DNA sequence among the isolates. Pasteurella multocida is one of the most important Gram-negative bacteria. It is upper respiratory tract [or pharyngeal region] commensal of many animal species. However, under predisposing factors the organism becomes the etiological agent of a wide range of economically important diseases, such as fowl cholera in poultry, hemorrhagic septicemia in cattle and buffalo, atrophic rhinitis in swine and snuffles in rabbits

Comparison of alpha toxin gene among different types of Clostridium perfringens

Alpha toxin is found to be produced by all types of Clostridium perfringens. The genes encoding alpha toxin from the available five types of Clostridium perfringens [A I chicken strain], A [rabbit strain], B, C and D were PCR amplified using specific primers and the PCR products were examined on 1.5% [w/v] agarose gel and demonstrated the same bands comparable to the published alpha toxin gene. The PCR products of a toxin gene from the five types were separately sequenced and aligned with all published a toxin genes of Clostridium perfringens. Identities among all studied alpha toxin gene sequences and with the published ones were nearly 96-98%. There are no any significant differences among these nucleotide sequences. It is concluded that a toxin gem; sequences among different types of Clostridium perfringens are similar and highly conserved