ABSTRACT
The incidence of P. multocida isolates isolated from 50 random samples collected from chicken suffering from respiratory manifestation was 48%, the random serological classification showed 4 isolates [A:5, A:8, A:9and D:2]. RAPD-PCR using 4 random primers separately was demonstrated to characterize, identify and determine the differences in the DNA sequence among the isolates. Pasteurella multocida is one of the most important Gram-negative bacteria. It is upper respiratory tract [or pharyngeal region] commensal of many animal species. However, under predisposing factors the organism becomes the etiological agent of a wide range of economically important diseases, such as fowl cholera in poultry, hemorrhagic septicemia in cattle and buffalo, atrophic rhinitis in swine and snuffles in rabbits
Subject(s)
Pasteurella multocida/isolation & purification , Chickens , Polymerase Chain Reaction/methods , Base SequenceABSTRACT
Alpha toxin is found to be produced by all types of Clostridium perfringens. The genes encoding alpha toxin from the available five types of Clostridium perfringens [A I chicken strain], A [rabbit strain], B, C and D were PCR amplified using specific primers and the PCR products were examined on 1.5% [w/v] agarose gel and demonstrated the same bands comparable to the published alpha toxin gene. The PCR products of a toxin gene from the five types were separately sequenced and aligned with all published a toxin genes of Clostridium perfringens. Identities among all studied alpha toxin gene sequences and with the published ones were nearly 96-98%. There are no any significant differences among these nucleotide sequences. It is concluded that a toxin gem; sequences among different types of Clostridium perfringens are similar and highly conserved