[Comparative study between Triage Micro Parasite Panel and Immunofluorescent Assay in diagnosis of Cryptosporidium Protozoan in Sohag Governorate]

The zoonotic intracellular protozoan parasite Cryptosporidium is highly infectious and as low as 30 sporulated oocysts can cause infection in healthy volunteers. Cryptosporidium oocysts are shed in large numbers in the faeces of infected people or animals. cryptosporidium oocysts are resistant to environmental conditions and are able to resist standard disinfection e.g. chlorination of drinking water. Stool samples of 72 persons [9-64 years old] in rural areas of Sohag Governorate, Egypt, attending the out patients clinic of cancer institute of Sohag Governorate [Ministry of Health] and complaining of diarrhoea [immunocompromized patients], were examined for detection of Cryptosporidium oocysts using modified acid-fast staining. Only 25 patients stool samples were positive for Cryptosporidium oocysts. Detection of Cryptosporidium oocysts using modified acid-fast staining is time consuming and need expert individual. The sensitivity and specificity of the test are improved by newer tools, such as immunofluorescent assays and antigen-capture enzyme-linked immunosorbent assays which are now commonly used in diagnostic laboratories. Polymerase-chain-reaction [PCR] - based techniques are available as research tests. In the present study the sensitivity of Direct Fluorescent Antibody [DFA] Test [100%] was found to be higher than that of Triage rapid qualitative enzyme immunoassay [EIA] [96%] and the specificity of both tests reached 100% as all members of the control group gave negative results

Aim of the work: Comparison between Triage Micro Parasite Panel and Immunofluorescence Tests in diagnosis of Cryptosporidium protozoan in Sohag Governorate

Relationship between Enterobius Vermicularis infection and pelvic inflammatory diseases in children at Sohag Governorate, Egypt

A cohort of children presented with pelvic inflammatory diseases [gastro-intestinal and/or genitourinary surgical conditions] and had concomitant infection with E. vermicularis. To find out this relationship, a total of 135 patients suffering from different gastro-intestinal and genitourinary surgical conditions were selected from Departments of Surgery and Gynecology. They were subjected to stool analysis, peri-anal swabs and blood examination mainly for eosinophilia. The results showed that 26 patients out of 135 [19.2%] had. E. vermicularis with high eosinophilia in 30 cases [22.2%]. Identification of E. vermicularis by peri-anal swab test in the cohort was successful [16.30%]. Males were more affected than females with ratio 2.2:1 and age mean 7.13 +/- 1.92. As to residence and housing, rural children with bad housing [73.08% and 88.46% respectively] were more affected than those living in urban areas with healthy housing [26.92% and 11.54% respectively]

Comparative study between the effect of nitazoxanide and paromomycine in treatment of cryptosporidiosis in hospitalized children

Ninety children infected with Cryptosporidium parvum attending Al Azhar University Teaching Hospital [Assuit] were chosen [60 males and 30 females] with age range from 6 months to ten years. The patients were divided into two groups of 45 patients for each [G1 and G2]. All patients suffered from chronic diarrhea for more than fifteen days. Cross-matched 45 children suffering from chronic diarrhea were used as a control group [G3]. Stool samples were collected and examined for detection of Cryptosporidium oocysts using Sheather's sugar and Modified Ziehl-Nelseen stain techniques. The first group [G1] received Nitazoxanide [100 mg and 200 mg every 12 hours for 3 days for children aged 6 months to 3 years, and children aged 4 to 10 years respectively], G2 received Parornomycin [25mg/kg/day for 2 weeks]. Third group received placebo. Significant improvement and shortening of the duration of diarrhea occur in G1; of 45 patients received Nitazoxanide 39 cases showed complete clinical and laboratory cure [86.6%], 5 cases showed clinical improvement with reduction in the number of oocysts and 1 case showed no cure. In G2 of 45 cases received Paromomycin 31 cases showed complete cure [68.8%], 8 cases showed clinical improvement with reduction of oocysts number and 6 cases were not cured. Nitazoxanide proved highly effective than Paromomycin in cryptosporidiosis

Evaluation of a modified surgical technique for correction of vaginal and uterine prolapse in bovine

This study was carried out on 81 cows [48 vaginal and 33 uterine prolapses] and 83 buffalo-cows [53 vaginal and 30 uterine prolapses] belonging to 8 commercial dairy farms located in Beni-Suef, El-Menia and El-Fayoum provinces in the period from January 2006 to December 2008. A modified surgical technique and epidural analgesic regimen were applied on the investigated animals and compared with the field traditionally used technique. A total of 81 cases of prolapses from 3330 calveings [243%] were recorded, 48 cases [1.44%] of them were vaginal prolapse and 33 cases [0.99%] were uterine prolapse. Concerning buffalo cows, out of 1680 calvings, a total of 83 cases [4.94%] had prolapses, including 53 cases [3.15%] vaginal prolapse and 30 cases [1.78%] uterine prolapse. The incidence of reproductive tract prolapse was higher in multiparous aged cows [93.75% and 87.88% vaginal and uterine prolapse, respectively] than in primiparous cows [6.25% and 12.12% vaginal and uterine prolapses, respectively] and also higher in cows with poor body condition scores [1.66% vaginal and 1.10% uterine] than in well fed animals with good body condition score [1.05% vaginal and 0.91% uterine]. Buffalo cows showed the same trend. About 4 5.83% of cows with vaginal and 33.33% of cows with uterine prolapse were previously suffered from vaginal prolapse, while only 16.66% of cows with vaginal and 12.12% of those with uterine prolapse were previously suffered from uterine prolapse. This indicated that uterine prolapse is not a heritable condition, and it is not likely to reoccur, and there is no need to cull the cow as long as she breeds back, controversial to vaginal prolapses that reoccur and are heritable and needs culling of the cow and her heifer offspring. The investigated fertility parameters in unaffected animals were better than females affected with prolapses, although better non significant differences were detected with modified method than traditional ones. The incidence of postoperative complications in groups of animals treated with the modified technique were significantly [p<0.01] lower than in animals treated with traditional technique. Concerning the survival rate of the affected animals, higher percentages were recorded after using the modified technique. In general, the prognosis was favorable for uncomplicated cases. In conclusion, early hygienic interference and prompt treatment using the modified technique may be imperative to improve the prognosis by reducing the postoperative complications and increasing the future fertility of affected animals