Investigations on the glucosinolates of lepidium sativum growing in Egypt and their biological activity

Fractionation of the glucosinolate contents of both the seeds and fresh herb of Lepidium sativum growing in Egypt were carried out. The study of the glucosinolate contents of L. sativum seeds revealed the isolation and identification of glucotropaeolin and 2- Phenyl ethyl glucosinolate while the study of glucosinolate contents of the fresh herb revealed the presence of 2- ethyl butyl glucosinolate, methyl glucosinolate, butyl glucosinolate and glucotropaeolin. The identification of the isolated glucosinolates were substantiated through using different chemical methods [enzymatic hydrolysis] and spectroscopic determinations [UV, MS and GC-MS for the corresponding isothiocyanates]. Acute toxicity studies of pet ether and alcoholic extracts of aerial parts of the plant showed that the alcoholic extract is more safe than that of pet ether extract and both extracts have a hepatoprotective activity on liver at the same concentration [50 micro g/ml]. The different extracts of the seeds and herbs of Lepidium sativum showed a potent effect against the White fly [Bemisia tabaci]. The total glucosinolate and in particular glucotropaeolin showed significant activity against the pest, which gave a highest mortality percentage on the adult stage

Biological and insecticidal effects of total glucosinolates and different extracts of carrichtera annua [L.] DC. and farsetia aegyptia turra. on spodoptera littoralis [Boisd.]

Different extracts of Carrichtera annua and Farsetia aegyptia as well as theirglucosinolates were prepared and the efficacy of these extracts and totalglucosinolates was studied against the Egyptian cotton leafworm, Spodoptera littoralis. Aqueous extracts and total glucosinolates of both plants wereremarkably toxic against Spodoptera littoralis larvae. They exhibited strongantifeedant effect against the 4th instar larvae and proved to be the mostpotent extracts as compared to all other tested ones, where the ovipositionalrepellent effect reached 100%. Larval growth and development weresignificantly affected and no pupae were developed