Detection of Coxeilla brunetii in bulk tank milk samples from dairy bovine farms using nested-PCR in Qom, Iran, 2011

Q fever is a zoonotic disease caused by Coxiella burnetii, a species of bacteria that is distributed worldwide. In cattle, Coxiella burnetii infections are generally asymptomatic but can also be associated with reproductive disorders. The aim of this study was to achieve molecular detection of Coxiella burnetii in dairy bovine milk farms using Nested PCR in Qom province, Iran. From January to February 2011 [winter] and July to September 2011[summer] a total of 100 bovine bulk milk samples were equally collected from five areas of Qom. The nested PCR assay used to screen for C. burnetii was designed from the nucleotide sequence of the com1 gene encodin a 27-kD outer membrane protein [OMP]. In this study, 14% [14 of 100] of bulk milk were positive. These results support the hypothesis of high prevalence and endemic pattern of Q fever in Qom province of Iran

[Evaluation of 4-Amino salicylic acid chelating effect on healing of manganese induced intoxications in male Wistar rat's liver]

Manganese related jobs [Mn miners] may cause Manganism, a Parkinson like disease. Chelators are suggested to reduce these effects. This study was carried out to investigate the effect of 4-Amino Salicylic acid chelating effects. This experimental study was done on 20 adult male Wistar rats. The rats were classified into four groups, 5 in each group, as follows: one control and three experimental groups. In two phases the groups received Salin-Salin, Manganese-Salin, Salin-4-Amino acid and Manganese -4-Amini acid, respectively. Eight mg/kg of manganese chloride i.p. injection for 1 week and 1.5g/l of PAS s.c injection five-day in a week for four weeks, were used for intoxication and treatment, respectively. Serum and liver tissues were separated. Data were analyzed using ANOVA and Tukey's tests. Biochemical factors showed cholesterol increment in group 2 compared with group 1 and decrement of cholesterol level in the blood of group 4 compare with group 2 [P<0.01]. Also increasing the direct billirubin in the Mn and PAS groups was more significant compared with control group [P<0.05]. Billirubin was observed in the urine of the Mn, PAS, Mn+PAS groups and urobilinogen declining was decreased in the urine of these groups [P<0.05]. There was a significant increase [P<0.05] of OT, PT enzymes in the serum of PAS and ALP in the Mn and Mn+PAS groups compared with control group. PAS can play a significant role in reducing destructive effects of Mn, especially on liver but has different side effects, like other chemical drugs