ABSTRACT
Sixty cows and 40 buffalos were diagnosed clinically repeat breeder animals. Uterine samples were collected and examined bacteriologically. The sensitivity of bacterial isolates recorded from the infectious cases for the antibiotics was studied. The microorganisms isolated from the uterine samples of cows and buffalos were Strept. bovis [5% and 7.5%], Staph. epidermidis [4% and 18.8%], Strept. facium [10.3%, 7.7%], Corynebacterium [18.1% and 14.8%], E. coli [6% and 1.0%], Enterobacter [10.6% and 8.9%], Pseudomonas [1.0% and 6.1%], and unclassified species [17.5% and 19.8%] respectively. Several medicaments were used to treat the repeat breeder animals symptomatically or based on the results of the bacterial sensitivity tests. Lugol's iodine 0.5%, lotagen 4%, mastalone and the animal own plasma were used based on symptoms. Tetracycline, ampicillin and sulfonamide were used to treat the repeat breeder animals in two ways. The first without sensitivity tests and the second based on sensitivity tests against the isolated bacterial strains. The results indicated that the most popular causes of repeat breeder were endometritis [40%] and cervicitis [30%] for cows and cervicitis [35%] and endometritis [25%] in buffalos. The results revealed that the high conception rate was obtained following the treatment of the animal with its own plasma [71.4% and 80%] and the ampicillin [50% and 50%] without sensitivity test for cows and buffalos, respectively. According to the results of the sensitivity tests, the best medicaments were tetracycline [60% and 60%], sulfonamide [50% and 66.6%] and ampicillin [75% and 100%] for cows and buffalos respectively. It was clear that the endometritis and cervicitis were the most popular causes of repeat breeder syndrome in cows and buffalos that were found with small holders. As a new trend, it was valuable to use the animal own plasma to treat such syndrome. However, the use of antibiotics will be more effective if used on sensitivity tests bases