ABSTRACT
The aim of this study was to conduct a sero-epidemiological survey in Meshkinshahr, Ardabil Province, northwestern Iran to detect the rate of hydatidosis in the city and nearby villages. Literature shows that no such study has been conducted so far. Overall, 670 serum samples were collected from 194 males and 476 females from patients referred to different health centers of the region. All patients filled out a questionnaire and an informed consent. Sera were analyzed using indirect-ELISA test. Ten micro g/ml antigens [Antigen B derived from hydatid cyst fluid], serum dilutions of 1:500 and conjugate anti-human cocombs with 1:100000 dilutions were utilized to perform the test. Data analysis was conducted using SPSS software ver. 11.5. The seroprevalence of human hydatidosis was 1.79% by ELISA test in the region. This rate for females was 1.68% and males 2.6%, respectively. There was no significant difference as regards all factors studied and the seropositivity. According to job, farmers and ranchmen had the highest rate of infection as 3.17%. The sero-prevalence of infection was 2.6%, respectively. There was no significant difference as regards all factors studied and the seropositivity. According to job, farmers and ranchmen had the highest rate of infection as 3.17%. The sero-prevalence of infection was 2.6% in illiterate people which showed the highest rate. As regards residency, urban life showed no significant difference with rural life [1.1% vs. 2.58%]. Age group of 69-90 yr old, with 4.62 as prevalence had the highest rate of positivity. Obtained sero-prevalence of hydatidosis shows more or less a resemblance to other cities of Iran, although due to the specific condition of the city we expected more rate of sero-positivity
ABSTRACT
In this study, we used both ITS1 and ITS2 for molecular identification of Fasciola species. The region between 18S and 28S of ribosomal DNA was used in PCR-RFLP method for molecular identification of Fasciola species. Ninety tematodes of Fasciola were collected during abattoir inspection from livers of naturally infected sheep and cattle from Khorasan, East Azerbaijan, and Fars provinces in Iran. After DNA extraction, PCR was performed to amplify region ITS1, 5.8S rDNA, ITS2. To select a suitable restriction enzyme, we sequenced and analyzed the PCR products of F. hepatica and F. gigantica samples from sheep and cattle. Tsp509I fast digest restriction enzyme was selected for RFLP method that caused the separation specifically of Fasciola species. The fragment approximately 1000bp in all of the Fasciola samples was amplified and then digested with the Tsp409I restriction endonuclease. Seventy F. hepatica and 20 F. gigantica were identified of total 90 Fasciola isolates. The new PCR-RFLP assay using Tsp509I restriction enzyme provides a simple, practical, fast, low cost, and reliable method for identification and differentiation of Fasciola practical, fast, low cost, and reliable method for identification and differentiation of Fasciola isolates
ABSTRACT
Due to scarcity of human reports, we took advantage of the heaviest infection of M. moniliformis in rats, to describe histopathological and microanatomical valuable useful keys while confronting human occurrences. Samples were obtained from captured rats in Tehran, capital of Iran, during two decades. Tissues sections were performed through hematoxylin and eosin staining to describe histopathological changes in rat's intestines. Totally, nine rats were found infected with M. moniliformis amongst 272 obtained rats. Heavy infection has been distinguished in 2 individuals with parasite burden of 141 and 73 adult worms. Cross sections of worms within the lumen show mucosal thickness, infiltration of eosinophilic leukocyte and increase in goblet cells. Beyond the uncommonness of human infection with M. moniliformis unintended infections should not be ignored. Abundance of rats and roaches as definite and intermediate hosts must be considered particularly in countries with poor hygiene
ABSTRACT
Intestinal parasitosis has been a major public health problem in Iran. It is necessary to evaluate an up-date data in this regard to be used by local authorities. Serum IgE determinations and coproparasitological analyses were conducted on 1200 individuals in city of Ghaemshahr, Mazandaran province, northern Iran. A total of 1200 subjects were taken stool samples for three days consecutively. Two hundred and ninety seven cases [24.7%0] were positive for parasitic diseases. The age group of 8-15 years old encompassed the highest rate of infection which showed a significant difference with the other groups [P< 0.01]. No significant difference was detected between males and females as to the rate of infection based on the Chi-squared test [14.2% vs. 10.6%], but a significant difference was seen between infection rate and cases education [P<0.01]. An about 5-fold elevation in serum IgE level was demonstrated. Intervention programs including health education and environmental sanitation are recommended