ABSTRACT
Recent literatures show that accelerated Portland cement [APC] and calcium hydroxide Ca [OH]2 may have the potential to promote the bone regeneration. However, certain clinical studies reveal consistency of Ca [OH]2, as one of the practical drawbacks of the material when used alone. To overcome such inconvenience, the combination of the Ca [OH]2 with a bone replacement material could offer a convenient solution.To evaluate the soft tissue healing and bone regeneration in the periodontal intrabony osseous defects using accelerated Portland cement [APC] in combination with calcium hydroxide Ca [OH]2, as a filling material.Five healthy adult mongrel dogs aged 2-3 years old [approximately 20 kg in weight] with intact dentition and healthy periodontium were selected for this study. Two one-wall defects in both mesial and distal aspects of the 3rd premolars of both sides of the mandible were created. Therefore, four defects were prepared in each dog. Three defects in each dog were randomly filled with one of the following materials: APC alone, APC mixed with Ca [OH]2, and Ca [OH]2 alone. The fourth defect was left empty [control]. Upon clinical examination of the sutured sites, the amount of dehiscence from the adjacent tooth was measured after two and eight weeks, using a periodontal probe mesiodistally. For histometric analysis, the degree of new bone formation was estimated at the end of the eighth postoperative week, by a differential point-counting method. The percentage of the defect volume occupied by new osteoid or trabecular bone was recorded Measurement of wound dehiscence during the second week revealed that all five APCs had an exposure of 1-2 mm and at the end of the study all samples showed 3-4 mm exposure across the surface of the graft material, whereas the Ca [OH]2, control, and APC + Ca [OH]2 groups did not show any exposure at the end of the eighth week of the study. The most amount of bone formation was observed in APC group which was significantly different with all other groups [p < 0.05].Despite acceptable soft tissue response of Ca [OH]2, this additive material could not be suggested because of negative effects on bone formation results
ABSTRACT
Statement of problem: Salivary gland neoplasms show varied histological and biological characteristics, and there may be difficulty in differentiating between low grade malignancies and benign tumors of the salivary glands
Purpose: The aim of this study was to investigate the diagnostic value of modified AgNOR technique in distinguishing between benign and malignant salivary gland tumors
Materials and Method: In this cross-sectional study, 40 benign salivary gland tumors [34 pleomorphic adenoma, 6 warthin tumor], 40 malignant neoplasms [26 adenoid cystic carcinoma, 14 mucoepidermoid carcinoma] and 10 chronic sialadenitis were stained with modified AgNOR technique. AgNOR counts, variation in size and dispersion of AgNOR dots in the cells were graded and compared in benign and malignant tumors. The data were statistically analyzed by ANOVA, Tukey, t-test and Kruskal-Wallis tests
Results: The mean AgNOR counts for the benign neoplasms [1.29+/-0.13] were less than those for the malignant neoplasms [2.87+/-0.25]. Variation in AgNOR size and dispersion was of a higher grade in malignancy when compared with benign neoplasms [ p <0.05]
Conclusion: Our results suggest that modified AgNOR staining is useful for distinguishing benign from malignant tumors, but variation in AgNOR size and dispersion in addition to the count should also be considered