VALIDITY OF LOCATION OF MUSCULAR UNITS ESTIMATED THROUGH INVERSE ANALYSIS OF SURFACE ELECTROMYOGRAMS / 体力科学

We have developed a method for estimating the depth and intensity of muscular unit represented as equivalent current dipoles by the inverse analysis of surface electromyograms (EMGs) . In this study, the validity of the locations of current dipoles estimated through the inverse analysis was verified by animal experiments. Surface motor unit action potentials (MUAPs) were recorded from the gastrocnemius muscle activated by electrical stimulation at the ventral root of lumbar spinal cord (L4 or L5) of rats. After recording the surface MUAPs for the inverse analysis and glycogen depletion of active muscle fibers by repeated electrical stimulation, periodic acid-Schiff (PAS) staining was used to determine the position of muscle fibers belonging to an active single motor unit. In the results of the inverse analysis, the values of ‘goodness of fit’ between measured and calculated MUAP were 71%, 79% and 85%. Estimated depths of current dipoles ranged from 1.8 mm to 5.9 mm. The locations estimated through the inverse analysis were more medial and shallower than the actual distribution of active muscle fibers determined by PAS staining. These errors were probably caused by the effects of the boundary in the model, the relationship between the measurement area and the location of an active motor unit, and the artifacts such as deformation of the muscle during dissection and freezing.

MUSCULAR UNIT SIZE AND FIBER DENSITY DEDUCED FROM SIMULATION OF INVERSE ANALYSIS OF SURFACE ELECTROMYOGRAMS / 体力科学

The purpose of this study was to noninvasively extract information about the size and muscle fiber density of muscular units through the inverse analysis of surface electromyograms. Surface motor unit action potentials (MUAPs) were recorded with a multi-channel electrode array arranged along the circumference direction of the biceps brachii. The depth and intensity of equivalent current dipoles were estimated through the inverse analysis of surface MUAPs. The simulation of inverse analysis of surface potentials generated by the muscular unit models showed that the relationship between the depth and the intensity depends on the muscular unit size and muscle fiber density.<BR>In the simulation, we systematically varied the model parameters including distance from the skin, radius, and fiber density and used the inverse analysis to estimate the depth and intensity of current dipoles. And, our method to estimate the radius and fiber density of muscular units using estimated depth and intensity is demonstrated. Mean values (± SD) estimated from the surface MUAPs were 3.0±1.8mm for depth and 13.8±32.0nAm for intensity. The estimated distance ranged from slightly less than 1 mm to slightly more than 2 mm. The estimated radius ranged from 1.8 to 4.6 mm and fiber density from 0.7 to 5.4 fibers/mm<SUP>2</SUP>.

FATIGABILITY OF MOTOR UNITS IN FIRST DORSAL INTEROSSEUS MUSCLE EVALUATED USING COLLISION METHOD / 体力科学

We evaluated motor unit (MU) fatigue in the first dorsal interosseous muscle (FDI) using the collision principle. Eight healthy men exerted 70% (short-duration fatigue task: SDF task) and 30% (long-duration fatigue task: LDF task) maximum voluntary contraction of isometric abductions in the left FDI until exhausted. Before and after voluntary contractions, the ulnar nerve was stimulated at the wrist and elbow with supramaximal intensity, and a pair of M-waves was obtained. Fatiguerelated changes were studied in mean power frequency (MPF), averaged rectified value (ARV) calculated from surface EMG, and motor nerve conduction velocity (MCV) and distribution of motor nerve conduction velocity (DMCV) calculated from M-waves. The MPF of voluntary EMG decreased, whereas ARV increased significantly during SDF and LDF tasks, indicating fatigue had developed in the FDI. Endurance was significantly shorter in the SDF task than in the LDF task (p<0.01), whereas differences between tasks were not seen in MPF and ARV changes. Tasks did not affect MCV, but lower components in DMCV increased for both tasks. Increased lower components were larger in the LDF task than in the SDF task. The shift in DMCV indicated that fatigued MUs stopped activity and enduring MUs, which had lower axon conduction velocity, were activated selectively. These results suggest that the collision principle is applicable in evaluating motor unit fatigability.

Effect of Long-Term Exercise on Walking Ability in Elderly People / 体力科学

In order to clarify the effect of exercise on the walking performance and the muscle volume in lower limbs, elderly athletes long continuing to be trained and untrained elderly were compared with regard to their muscle cross-sectional area (CSA) of m. psoas major, thigh muscle and crus muscle and their walking ability. The subjects used consisted of thirty-six 80's-aged male and 70's-aged female elderly athletes and twenty-four elders having no regular exercise (control male group : CM, control female group : CF) . The elderly athletes were further divided into two groups in accordance with their results of Japan Fitness Test (high performance male group : HPM, low performance male group : LPM, high performance female group : HPF, low performance female group : LPF) . The walking performance was evaluated by analyzing their walking speed, stride-length and step rate during walking along a 15 m-strip of passage at normal and fast paces using videotaping. The muscle CSA was determined at m. psoas major, thigh muscle (extensors and flexors) and crus muscle (m. tibialis anterior and m. triceps surae) using MRI. As for the walking speed and stride-length at the normal pace, only HPM and HPF showed significantly higher values than CM and CF (male : p<0.05, female : p<0.01) . Meanwhile at the faster pace, HPF and LPF showed significantly higher values than CF in female (HPF : p<0.01, LPF : p<0.05) and in the case of males, only HPM have a higher value only of the walking speed than CM (p<0.05) . The CSA of m. psoas major in HPM and HPF significantly higher than that in CM and CF (all p<0.05), while in CSAs of knee extensor muscles and m. triceps surae, the statistical differences were not consistent among male and female groups. The results suggested that greater muscle mass of m. psoas major could influence higher walking speed in elderly people, and might be affected by regular exercise training.

Relationship Between Reduction of Hip Joint and Thigh Muscle and Walking Ability in Elderly People / 体力科学

The purpose of this study was to investigate the hypothesis that the reduction in walking ability is due to muscle atrophy in the lower limb muscles with aging using equational structure modeling as well as investigate the influence of muscle on walking ability. The subjects consisted of 127 persons (57 males and 70 females) aged 20-84 year, who were grouped into 6 age brackets of 20-39, 40-49, 50-59, 60-69, 70-74, and 75 or older. Using MRI, muscle cross-sectional area was measured on psoas major and thigh muscle (divided into extensor and flexor) . For walking patterns, each subject walked along a 7-m walking passage at normal speed for VTR-recording of the motion. The resulting pictures were used to analyze stride length, trunk inclination and walking speeds. Walking speeds showed a statistically significant decrease in value from the 50's age group in males and the 60's age group in females when compared with the 20-39 age bracket (p<0.05) . In males, a significant co-relationship was observed only between the muscle cross-sectional area of thigh extensor and walking speed (p<0.01) while in females, a significant co-relationship was found between the muscle cross-sectional area of psoas major (p<0.001) /thigh muscle extensor (p<0.01) and walking speed. These results indicate that the muscle atrophy with aging in psoas major and thigh muscle extensor is a factor responsible for the decrease in walking speed. Meanwhile, a difference in sex was observed between the muscle cross-sectional area of psoas major and walking speed. It was considered that the muscle atrophy rate of the female's psoas major being higher than the male's influenced this. Furthermore, it was suggested possibility that the decline of walking ability is due to decreased muscle mass of the lower limbs with aging.

The effect of strength training in muscle and nerve is memorized and reinforced by retraining / 体力科学

The purpose of this study was to prove the hypothesis that the effect of strength training is memorized and reinforced by retraining. Untrained university-age men participated in this training program. The retraining leg was subjected to 5 weeks of isometric training, 17 weeks of detraining and 5 weeks of retraining in knee extension. The contralateral training leg was subjected to 5 weeks of isometric training during the same period as the retraining phase of the retraining leg. Maximal isometric torque of knee extension increased after the 5-week training and remained at the trained level during the 17week detraining period. Torque gain by retraining of the retraining leg was 2.6 times greater than that of the contralateral training leg. These changes in isometric torque corres-ponded with changes in iEMG of the vastus lateralis. The cross-sectional area of the quadriceps femoris muscle did not change with training. Results support the hypothesis that the effect of strength training is memorized and reinforced by retraining. In addition, results show that these adaptations would be explained by recruitment and rate coding of motor units.

Evaluation of voluntary muscle activation and tolerance for fatigue using twitch interpolation technique / 体力科学

The purpose of this study is to examine the validity of muscle fatigue evaluation using maximum voluntary torque (MVT), and to identify the dependence of individual's tolerance for fatigue on the capacity to exert MVT. In 14 young male subjects (10 regular exercisers and 4 sedentary), MVT was measured during isometric knee extension, and voluntary activation (VA), which reflects motor unit activation, was evaluated using the twitch interpolation technique. In addition, the maximum endurance time (ET) was measured, and behavior of the mean power frequency (MPF) and the average rectified value (ARV) of surface EMGs from the vastus lateralis muscle were analyzed during constant force isometric contractions of 60% MVT (short-duration fatigue task; SDF task) and 20% MVT (long-duration fatigue task; LDF task) . Correlations were examined among these five variables.<BR>The results were as follows:<BR>1) Subjects were divided into a high voluntary activation group (HVA group) and a low voluntary activation group (LVA group) . Four sedentary subjects were included in the latter group.<BR>2) MVT was significantly larger in the HVA group than in the LVA group (p<0.01) . A significant positive correlation (r=0.72) was found between MVT and VA (p<0.01) .<BR>3) A significant negative correlation (r=-0.71) was found between MVT and endurance time (ET) for the LDF task (p<0.01) . The ET was significantly longer in the LVA group than in the HVA group (p<0.01) .<BR>4) The MPF of voluntary EMG decreased consistently, as ARV increased during isometric contraction in both tasks (p<0.01), indicating the development of fatigue in the muscle. The final change of MPF relative to the initial value was significantly greater in the SDF task than in the LDF task (p<0.05) .<BR>5) A significant correlation (r=-0.83) was seen between the relative change in MPF and ARV in the SDF task (p<0.01) .<BR>6) For the SDF task, the final change of MPF and ARV relative to the initial value was significantly greater in the LVA group than in the HVA group (p<0.05) .<BR>These results indicate that tolerance for local muscle fatigue usually evaluated as maximum endurance time, may depend on individual differences in VA, the VA, in turn, depending on adapta-tion to exercise, and that there appears to exist a corresponding adaptative strategy of the neuromuscular system during fatiguing contractions. Usefulness of our procedure using the twitch interpolation technique in evaluating muscle fatigue was also suggested.

Three-dimensional dynamics of elbow and shoulder joint throwing motion in relation to throwing arm movement and injury in baseball / 体力科学

This study was designed to clarify the causes of throwing injuries of the elbow and shoulder joints in baseball. Five varsity-skilled baseball players without pain in the elbow and shoulder joints were subjects for this study. They were fixed to a chair and asked to throw a baseball using three different throwing arm movements (T<SUB>0</SUB>, T<SUB>45</SUB>, and T<SUB>90</SUB>) . These movements were filmed using three-dimensional DLT videography. Linked rigid-body segment inverse dynamics were then employed to determine resultant joint force and torque at the elbow and shoulder joints. Peak varus torque at the elbow joint for T<SUB>90</SUB> was less than for the other movements during the acceleration phase. In the follow-through phase, however, a large anterior shear force (70 N) at the elbow, for elbow extension, was present for T<SUB>90</SUB>. These results indicate that T<SUB>90</SUB> was a high risk movement which leads to extension injuries rather than medial tension injuries. After the ball release, a large superior shear force (118 N) at the shoulder joint was present in all movements. This superior force may result from the subacromial impingement syndrome, except for critical zones of impingement caused by the different throwing arm movements. These findings suggest that the mechanisms of throwing arm injuries are closely related to differences in throwing arm movements.

Characteristics of muscle oxygenation in elderly men determined by near infrared spectroscopy / 体力科学

We used near-infrared spectroscopy (NIRS) to study noninvasively the effects of aging on changes in muscle oxygenation during steady bicycle exercise. For the study, 6 healthy young males and 13 healthy elderly male volunteers were recruited. To evaluate the physical fitness level and to determine exercise intensity, the ventilatory threshold (VT) was first measured. As a result, elderly subjects were divided into two groups according to O<SUB>2</SUB> uptake at VT (Elderly-H ; 936.0±26.4, Elderly-L ; 695.3±29.9, Young ; 790.0±51.19 ml) . Secondly we measured muscle oxygenation by NIRS at rest and during exercise at relative work intensities of VT ; 20%, 40%, 60%, 80% and 100%. In all cases muscle oxygenation at rest and during exercise was expressed as a relative value from 100% oxygenation (oxygen capacity) established by thigh occlusion (ischemia) . All subjects showed progressive deoxygenation with increasing intensity. There were no differences between the three groups in muscle oxygenation during exercise at relative work intensity of VT. These data suggest that aging and physical fitness level have no effect on muscle oxygenation below relative work intensity of VT.

Relationship between force level and exertion strategy in rapid isometric contractions / 体力科学

The relationships were examined between the relative force level and exertion strategy during rapid isometric contractions. Thirteen subjects exerted isometric pinchings as fast as possible at various force levels. Force curves and surface EMGs from the first dorsal interosseous muscle were obtained during the contractions. While the single-peaked force curves remained analogous up to a certain force level, they changed to multi-peaked ones at higher force levels. The critical force level, which could be determined in all subjects as relative force level to MVC, was positively correlated with the %MVC EMG magnitude for unit time, obtained below the critical level. EMG durations were significantly shorter blow than above the critical level. These results suggest that different exertion strategies are utilized in rapid contractions at different force levels, and that individual differences in the critical force level are related to difference in the manner of motor unit activation.

A Three-dimensional analysis on mechanical energy flows of torso and arm segments in baseball throw / 体力科学

Twenty-four male university baseball players were each requested to throw a baseball, and filmed using the direct linear transformation method of three-dimensional (3D) videography. 3 D coordinates of landmarks were obtained. Resultant joint forces and resultant joint torques in the wrists, elbows, shoulders, neck, and upper torso joints were calculated using the inverse dynamics method. The mechanical powers caused by the resultant joint forces (joint force power) and by the resultant joint torques (joint torque power) of each segment were calculated, and the mechanical work was also obtained by integrating the joint torque powers with time. Peak values of energies of the upper torso, upper arm, forearm, hand, and ball appeared in sequence from the proximal segment to the distal segment. The joint force powers in any segment were markedly larger than the joint torque powers. Little joint torque power was produced in the wrist throughout the throwing motion. The negative joint force power and joint torque power at the proximal end of the upper torso were rapidly increased immediately after the foot contact stride. It was clarified that the appearance of the large energies in the distal throwing arm segments during the final phase of throwing motion were caused mainly by transfer of the energies produced by the motions of the torso and shoulder joints. This paper discusses the mechanical energy flows of the upper torso and upper limb segments during the motion of baseball throwing.