Research hotspots of diabetes management in China / 预防医学

Objective@#To analyze the research status,research hotspots and its changing trends of diabetes management in China. @*Methods@#We searched the databases of CNKI,Wanfang and VIP to collect literature about diabetes management until December 7th of 2019;counted the number of published articles and journals;plotted the co-occurrence maps of authors and institutions,and time zone map of keywords by CiteSpace software,so as to analyze the research status,research hotspots and its changing trends of diabetes management.@*Results @#A total of 8 435 articles were included,and the number of the articles was increasing year by year. The largest number,8 141 (96.51%) of published articles lay in the period from 2009 to 2019. The articles published in 826 journals,13 of which were in the core area. Diabetes New World published the largest number,419 (4.97%) of articles. Lou Qingqing was the author with the most articles published (26,accounting for 0.31%). Shanghai Jiao Tong University,West China Hospital of Sichuan University and the First Affiliated Hospital of Jinan University ranked the top three in articles published,with 70 articles accounting for 0.83%. The keywords that appeared more frequently were self-management,health education,blood glucose management,gestational diabetes,health management,community management and nursing management. The more centralized keywords were self-management,health education,gestational diabetes and community management. Self-management and health education had always been research hotspots. Family doctors and mobile health were new directions in recent years.@*Conclusions@#The volume of domestic articles on diabetes management has been increasing. Self-management and health education has always been hotspots,meanwhile,family doctor and mobile health have become new hotspots of diabetes management research.

AIDS discrimination in junior college students and the effect of AIDS knowledge on discrimination / 中国学校卫生

Objective@#To analyze the situation of AIDS knowledge and discrimination among freshmen in Chengdu city, and to explore possible effects of AIDS knowledge on discrimination.@*Methods@#A cluster random sampling was employed to investigate 1 053 college students from 11 universities in Chengdu about their HIV/AIDS knowledge and discrimination. The scores of AIDS knowledge and discrimination of students with different characteristics were analyzed, and the influence path of AIDS knowledge on AIDS discrimination were further analyzed based on different peer relationships.@*Results@#The total scores of AIDS knowledge was negatively correlated to AIDS discrimination( r s =-0.13, P <0.01). After adjusting for confounding factors, the total score of AIDS knowledge was associated with the total score of AIDS discrimination( β =-0.12, P <0.01). AIDS knowledge played a role in AIDS discrimination in intimate, general and unfamiliar peer relationships, with standardized path coefficients of -0.20, -0.24 and -0.18 respectively( P <0.01).@*Conclusion@#AIDS knowledge are correlated with discrimination among freshmen under different peer relationships. More anti-AIDS discrimination courses should be added to AIDS education to reduce the students’ fear and stigma of HIV/AIDS patients and related risk groups.

Case-control study on the relationship of chronic low back pain of facet joint origin with the distribution of nerve endings and neuropeptide: a quantitative histological analysis / 中国骨伤

<p><b>OBJECTIVE</b>To investigate the density and distribution of nerve endings and neuropeptide Y (NPY) in lumbar facet joints of patients with low back pain.</p><p><b>METHODS</b>Fifteen patients without low back pain were selected as control group (group A). Facet joint samples in group A were obtained during the operation or lumbar spinal canal tumor they suffered from. Those patients with low back pain were divided into three groups according to their different origins of pain, such as not from facet joint (group B, 15 patients) ,from facet joint only (group C, 20 patients), or from facet joint partially (group D, 20 patients). Different origins were determined by VAS after facet joint block. The density and distribution of nerve ending and neuropeptide in the capsular tissues were analyzed by a modified gold chloride staining and immunochemistry respectively.</p><p><b>RESULTS</b>Compared with the ones in group A and B, the fact joints in group C and D were more inclined to be degenerated and got more nerve endings. NPY was expressed mainly in the facet joint of patients with low back pain in group C and D. In addition, there was a significant relationship between the distribution of nerve endings and NPY expression,while none of them were related with MRI Fujiwara grade of facet joint.</p><p><b>CONCLUSION</b>These results suggest that the number of mechanoreceptors, neural sprouting and secreted peptides in the facet joint capsules vary with the change of mechanical or nociceptive stimulation, which may promote the development of low back pain in return.</p>

Effect of acupuncture on the activity of the peripheral blood T lymphocyte subsets and NK cells in patients with colorectal cancer liver metastasis / 中国针灸

<p><b>OBJECTIVE</b>To study the effect of acupuncture on the immune function of patients with colorectal cancer liver metastasis.</p><p><b>METHODS</b>Sixty cases with colorectal cancer liver metastasis confirmed by pathology and mageology diagnosis were treated with acupuncture. Zusanli (ST 36), Sanyinjiao (SP 6), Neiguan (PC 6), Shangjuxu (ST 37), Hegu (LI 4), Taixi (KI 3), Taichong (LR 3) ,Yinlingquan (SP 9), Yanglingquan (GB 34), etc. were selected for acupuncture, and Shenque (CV 8), Guanyuan (CV 4), Qihai (CV 6), Zusanli (ST 36) were selected for moxibustion. The changes of CD(3) , CD(4) , CD(8) T cells and NK cells in value were examined with flow cytometry before and after treatment.</p><p><b>RESULTS</b>The value of T lymphocyte subsets such as CD(3) , CD(4), and CD(8) , as well as NK cells were obviously increased after treatment, and there were significant differences between them before and after treatment.</p><p><b>CONCLUSION</b>Acupuncture can improve the immune function of patients with colorectal cancer liver metastasis.</p>

LIPUS enhance elongation of neurites in rat cortical neurons through inhibition of GSK-3beta / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>Low-intensity pulsed ultrasound (LIPUS) has been reported to enhance proliferation and to alter protein production in various kinds of cells. In the present study, we measured the neurites length after LIPUS treatment to define the effectiveness of LIPUS stimulation on neurons, and then we examined the acticity of GSK-3beta to study the intracellular mechanism of neurite's outgrowth.</p><p><b>METHODS</b>LIPUS was applied to cultured primary rat cortical neurons for 5 minutes every day with spatial- and temporal average intensities (SATA) of 10 mW/cm(2), a pulse width of 200 microseconds, a repetition rate of 1.5 KHz, and an operation frequency of 1 MHz. Neurons were photographed on the third day after LIPUS treatment and harvested at third, seventh, and tenth days for immunoblot and semi-quantitative RT-PCR analysis.</p><p><b>RESULTS</b>Morphology change showed that neurite extension was enhanced by LIPUS. There was also a remarkable decrease of proteins, including p-Akt, p-GSK-3beta, and p-CRMP-2, observed on the seventh and tenth days, and of GSK-3beta mRNA expression, observed on the seventh day, in neurons treated with LIPUS.</p><p><b>CONCLUSION</b>LIPUS can enhance elongation of neurites and it is possible through the decreased expression of GSK-3beta.</p>

Protective effect of uridine 5′-triphosphate on brain tissue after cerebral ischemia-reperfusion injury in rats / 第二军医大学学报

Objective: To use MRI method for continuous observation of the protective effect of uridine 5′-triphosphate (UTP) against cerebral ischemia-reperfusion (IR) injury in rats. Methods: The cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) in rats. UTP solution was delivered through an indwelling tail venous catheter via microinfusion pump 30 min after MCAO at a rate of 5 ml·kg-1·min-1. Neurological deficit score (NDS) and brain water content were determined 24 h after reperfusion. The infarction volume was determined by 2,3,5-triphenyl- tetrazolium chloride (TTC) staining and MRI at 6, 30, and 54 h after reperfusion. Results: The NDS scores in 10, 30, and 90 μg/kg UTP groups were significantly lower than that in the saline control group (P<0.01); the water contents in the right cerebral hemisphere of 10, 30, and 90 μg/kg UTP groups were significantly less than that in the normal saline group (P<0.01). The cerebral infarction volume was gradually decreased with the increase of UTP dose, and the proportions of infarction volumes in 10, 30, and 90 μg/kg UTP groups were significantly decreased compared with the saline control group (P<0.01). The infarction volumes at 6, 30, and 54 h after reperfusion in 90 μg/kg UTP group were significantly lower than those in the saline control group (P<0.01). Conclusion: UTP has a protective effect on focal cerebral ischemia-reperfusion injury in rats; it can greatly reduce the volume of cerebral infarction.