Avaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral canina / Reliability evaluation between two evaluators in the cytopathologic and immunocytochemical exams from bone marrow aspirate in the canine visceral leishmaniasis diagnosis

Visceral leishmaniasis is a zoonosis in which the dog appears as the main source of infection in urban areas. Its diagnosis is complex and the cytopathological exam is a fast and cheap alternative to parasite direct visualization and its sensitivity can be increased by immunocytochemistry, though with a higher cost. The accuracy of such methods is dependent on the microscopist's experience and therefore, this study evaluated the reliability of such techniques between two observers, from bone marrow aspirates of 50 dogs from an endemic area for the disease. The parasitological culture in Novy-MacNeal-Nicolle medium was used as the reference standard. Among the main findings, the sensitivities obtained by observers I and II were respectively 62.5% and 37.5%, while specificities were 81.1% and 100%. On immunocytochemistry evaluation, the sensitivity was 0% for both evaluators and the specificity 97.3% and 100%. The agreement between evaluators was weak (κ = 0.167) for the cytopathological test and it could not be evaluated for immunocytochemistry, for which there was no detection by the evaluator II. The agreements among the diagnostic methods and the standard reference for the observer I were reasonable (κ = 0.364) for cytopathological examination and bad (κ = -0.041) for immunocytochemistry. For observer II, such agreement could be assessed only for the cytopathological test, being moderate (κ = 0.497). The results point to the possible expertise difference between evaluators, with the evaluator II demonstrating greater experience when interpreting the citopathological test. Although there was the expected sensitivity increase with immunocytochemistry, the technique used in this study was not effective for the diagnosis of infection, regardless of the evaluator.(AU)

Digestibilidade aparente dos nutrientes de dietas simplificadas baseadas em forragens para coelhos em crescimento / Apparent digestibility of nutrients of simplified diets based on forages for growing rabbits

Avaliaram-se os efeitos de dietas simplificadas à base de forragens sobre a digestibilidade aparente dos nutrientes em coelhos Nova Zelândia branco. As dietas experimentais foram: referência (REF), feno de alfafa (FAL), feno das folhas de rami (FRA), feno das folhas de amoreira (FAM) e feno do terço superior da rama da mandioca (FMA). A digestibilidade das dietas foi influenciada pelo tipo de alimento estudado; a FMA apresentou coeficientes de digestibilidade inferiores às demais dietas para todos os princípios nutritivos analisados. Para a dieta FAM, os coeficientes de digestibilidade aparente dos princípios nutritivos foram maiores (P<0,05) que os coeficientes das outras dietas estudadas. Os animais que ingeriram a dieta FAM apresentaram distúrbios digestivos e baixos consumos, com efeito negativo sobre os resultados. Os coeficientes de digestibilidade aparente das demais dietas estudadas foram semelhantes (P>0,05). Os valores estimados de energia digestível (kcal ED/kg MS) e proteína digestível ( por centoPD/MS) foram, respectivamente, para o feno de alfafa: 2285,27 e 16,04; feno das folhas de rami: 1857,88 e 16,37; feno das folhas de amoreira: 2838,48 e 15,12 e feno do terço superior da mandioca: 2155,55 e 10,57.

The effect of simplified diets based on forages on the apparent digestibility in white New Zealand rabbits was evaluated. The treatments were based on the following diets: reference (REF), hay of alfalfa (FAL), hay of rami leaves (FRA), hay of mulberry leaves (FAM) and hay of upper to 1/3 aereal part of cassava (FMA). The type of food affected the digestibility of the diets. The FMA diet showed low coefficients of digestibility in comparison to the other diets for all the analyzed nutrients. For the FAM diet the coefficients of apparent digestibility of the nutrients had higher values (P<0.05) than the coefficients of the other studied diets. The animals that ingested FAM diet showed digestive disorders and low consumption, with negative effect on the results. The coefficients of apparent digestibility of the remaining diets were similar (P>0.05). The estimated values of digestible energy (kcal DE/kg DM) and digestible protein ( percentDP/DM) were, respectively, 2285.27 and 16.04 for alfalfa hay, 1857.88 and 16.37 for hay of rami leaves, 2838.48 and 15.12 for hay of mulberry leaves and 2155.55 and 10.57 for hay of upper to 1/3 aereal part of cassava.