Interferon gamma and regulatory T-Cells and their relation with the pathological grading of gastritis caused by helicobacter pylori

Background: Helicobacter pylori [H.pylori] is a cause of chronic-active gastritis and a majority of cases of peptic ulcer disease. It is also associated with the development of gastric adenocarcinoma, the fourth most common malignancy in the world

Aim of the study: The study aims to detect the level of interferon gamma expression and the percentage of CD4+ CD25+ T regulatory cells in patients with gastritis and their relation to the pathological grading

Methods: Gastric biopsy specimens were taken from the antrum of 30 patiens using upper GIT endoscopy. Rapid urease test [RUT], immunohistochemistry [IHC], and Hematoxylin and Eosin [H and E] stains were used to determine H.pylori status. Evaluation of the histologic features was done using H and E. IFN-gamma mRNA expression in the gastric biopsies was measured using Real-Time PCR. Flowcytometry was used for measuring the percentage of CD4+CD25+regulatory T-ells [T regs] in the peripheral blood of all the patients. The results were compared to 15 healthy control subjects

Results: H.pylori was detected in 56.7% of all patients by RUT and in 93.3% patients by H and E and in 83.3% by IHC,out of them 88% had active gastritis and all of them had chronic gastritis. There was a statistically significant positive correlation between H.pylori positive infection and the pathological grading of active gastritis [p=0.015]. However, there was no statistically significant correlation between H.pylori infection [by IHC] and the pathological grading of chronic gastritis [p=0.334]. No statistically significant difference in the levels of IFN-gamma mRNA expression between H.pylori positive and negative patients was detected. No correlation was found between the levels of IFN-gamma mRNA expression and the severity of either active or chronic gastritis. Similarly, no correlation between the percentage of CD4+ CD25+ T regulatory cells and the severity of either active or chronic gastritis was detected

Conclusion: H.pylori plays an important role in active gastritis lesion. The correlation between IFN-gamma mRNA expression and the severity of active or chronic gastritis was overshadowed by the prevalence of helminthic infection among Egyptian patients. Meanwhile, low levels of T regulatory cells among H.pylori positive and negative gastritis than controls suggests an important role of T regulatory cells in regulating the gastric mucosal inflammatory response

Preterm deliveries and thier complications in El Matarey Teaching Hospital

The aim of the study was to evaluate the outcome of preterm deliveries in El-Matareya Teaching Hospital in the year 2001 and its complications on newborns. This is a retrospective study about the outcome and complications of preterm deliveries in El - Matareya Teaching Hospital in the year 2001.Preterm deliveries cause a significant increase in the neonatal morbidities and mortalities in comparison to full term deliveries

Protocol for management of recurrent pregnancy losses in the second and third trimester

The aim of the study was to evaluate the efficacy of the suggested management after recurrent pregnancy losses. This was retrospective study and it was done on pregnant women who previously failed to get living children. The results were so encouraging, all women who were managed by the suggested protocol got living children after recurrent pregnancy losses

Comparison between medical and surgical evacuation in early fetal demise

The aim of the study was to compare the results of medical and surgical evacuation in early fetal demise as regards efficacy and complications. The results insure that medical evacuation is an effective alternative option to surgical evacuation. A controlled prospective clinical trial. Medical evacuation is an option which may be safer than surgical evacuation especially in missed abortion or when evacuation was done by inexperienced doctor