Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add filters








Language
Year range
1.
Braz. j. med. biol. res ; 43(2): 195-200, Feb. 2010. graf
Article in English | LILACS | ID: lil-538230

ABSTRACT

Variations in the estrogenic activity of the phytoestrogen-rich plant, Pueraria mirifica, were determined with yeast estrogen screen (YES) consisting of human estrogen receptors (hER) hERá and hERâ and human transcriptional intermediary factor 2 (hTIF2) or human steroid receptor coactivator 1 (hSRC1), respectively, together with the â-galactosidase expression cassette. Relative estrogenic potency was expressed by determining the â-galactosidase activity (EC50) of the tuber extracts in relation to 17â-estradiol. Twenty-four and 22 of the plant tuber ethanolic extracts interacted with hERá and hERâ, respectively, with a higher relative estrogenic potency with hERâ than with hERá. Antiestrogenic activity of the plant extracts was also determined by incubation of plant extracts with 17â-estradiol prior to YES assay. The plant extracts tested exhibited antiestrogenic activity. Both the estrogenic and the antiestrogenic activity of the tuber extracts were metabolically activated with the rat liver S9-fraction prior to the assay indicating the positive influence of liver enzymes. Correlation analysis between estrogenic potency and the five major isoflavonoid contents within the previously HPLC-analyzed tuberous samples namely puerarin, daidzin, genistin, daidzein, and genistein revealed a negative result.


Subject(s)
Animals , Rats , Estrogen Receptor alpha/analysis , Estrogen Receptor beta/analysis , Liver/drug effects , Plant Extracts/pharmacology , Pueraria/chemistry , Biological Assay , Chromatography, High Pressure Liquid , Estradiol/metabolism , Estrogen Receptor alpha/metabolism , Estrogen Receptor beta/metabolism , Isoflavones/analysis , Isoflavones/metabolism , Liver/metabolism , Nuclear Receptor Coactivator 1/metabolism , /metabolism , beta-Galactosidase/analysis , beta-Galactosidase/antagonists & inhibitors
SELECTION OF CITATIONS
SEARCH DETAIL