ABSTRACT
There is considerable interest in alternative approaches for eradication of infections causes by pathogenic bacteria especially resistant bacteria. Methanol and ethanol extracts of Rumex alveollatus leaves were evaluated for in vitro antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa strains. In this empirical-experimental study, the agar plate well diffusion method was used for antibacterial assay of different sample. Broth microdilution method was used for evaluate Minimum inhibitory concentration [MIC] and Minimal bactericidal concentration [MBC] of extracts. All extracts demonstrated significant inhibitory effect against selected bacterial strains. There were statistically significant correlations between antibacterial activity and concentration of the extracts [p<0.05]. The MIC values of the extracts ranged from 31.3 mg/ml to 62.5 mg/ml whereas the MBC values ranged from 62.5 mg/ml to 125.0 mg/ml. Result from these finding suggest that methanol and ethanol extracts of Rumex alveollatus leaves may be used as natural antibacterial for treatment of some diseases, especially local skin diseases
ABSTRACT
Teucrium polium L. is a medicinal plant, which due to its antimicrobial, antispasmodic and anti-tumor properties has been used in traditional medicine for over 2000 years. The aim of this research was to study the effect of aqueous and ethanolic extracts of Teucrium polium L. against three strains of Candida albicans [ATCC 62061, ATCC 1677, and NCPF 3153], Malassezia furfur and Malassezia globosa using pour plate method. Teucrium polium L. was collected from Broojen area during the spring. The plant was dried and powdered. The aqueous and ethanolic extracts were prepared from the fine powder. Different concentrations of extracts [1, 2, 4, and 8 mg/ml] were made in Sabouraud Dextrose Agar [SDA] and modified Leeming-Notman Agar [MLNA] medium for Candida albicans, Malassezia furfur and Malassezia globosa. 1.5x10[6] cfu/ml of yeasts, were cultured on media and incubated at 37[degree]C and 32[degree]C respectively. Pour plate method was used to assess the antifungal activity of these extracts. The inhibitory effect of ethanolic extract of Teucrium polium L. on the three strains of Candida albicans was depended on concentration level of extracts in media. Aqueous extract had inhibitory effect on Candida albicans [NCPF 3153] only, and with increasing of the extract concentration, the number of colonies was decreased, so that in concentration of 8 mg/ml, no growth was seen. Aqueous and ethanolic extracts had no inhibitory effect on Malassezia species. Teucrium polium L. extracts have considerable inhibitory effect on different strains of Candida albicans. Further investigations are needed to detect the effectiveness of this plant in treatment of Candida infections