ABSTRACT
Leukemia is a blood disease that creates from inhibition of differentiation and increased proliferation rate. The nature has been known as a rich source of medically useful substances. High diversity of bioactive molecules, extracted from marine invertebrates, makes them as ideal candidates for cancer research. The study has been done to investigate cytotoxic effects of dichloromethane brittle star extract and doxorubic in on EL4 cancer cells. Blood cancer EL4 cells were cultured and treated at different concentrations of Brittle Star [Ophiocoma erinaceus] dichloromethane extract at 24, 48 and 72 h. Cell toxicity was studied using MTT assay. Cell morphology was examined using an invert microscope. Further, apoptosis was examined using Annexin V-FITC, propodium iodide, DAPI, and Acridine orange/propodium iodide staining. Eventually, the apoptosis pathways were analyzed using measurement of Caspase 3 and 9 activity. The statistical analysis was performed using SPSS, ANOVA software, and Tukey's test. P < 0.05 was considered to be significant. MTT assay and morphological observations showed that dichloromethane extract can inhibit cell growth in a dose dependent. The results considered 32 micro g/mL of the extract as IC[50]. Also, doxorubicin suppressed EL4 proliferation as IC[50]= 32 micro g/mL. All experiments related to apoptosis analysis confirmed that dichloromethane Brittle Star extract and doxorubic in have a cytotoxic effect on EL4 cells in IC[50] concentration. The study showed that dichloromethane Brittle Star extract is as an adjunct to doxorubicin in treatment of leukemia cells
ABSTRACT
Cancer is the second important reason of mortality in the world. In this regard melanoma was accounted as the most aggressive type of cutaneous cancer. Among drug extracted from natural products from marine organisms have been focused investigations related to chemotherapeutic agents derived from echinoderms such as sea cucumbers and starfish. In the present study, cytotoxic and apoptosis inducing potential of Persian Gulf brittle stars dichloromethane extract were evaluated against melanoma cancers. In this study, anti-proliferative effect of brittle stars dichloromethane extract on B16F10 melanoma cells examined by MTT assay and morphological characterization and death inducing effect of Annexin-PI and PI flow cytometry. The data analysis was performed by SPSS software and p<0.05 were considered significant. The dichloromethane extract of brittle star revealed significant cytotoxic effect on B16F10 melanoma cells with IC[50]= 31 microg/ml which is stronger than inhibitory effect of methanol extract on melanoma cell growth. In addition, brittle star dichloromethane extract recruited apoptotic pathway in the response of 31 microg/ml treatment. This study showed that certain concentrations of dichloromethane brittle stars possess cytotoxic activity that can be used as an anticancer agent used in clinical trial due to cell growth inhibition and apoptosis induction which offer therapeutic investigations of dichloromethane brittle star extract as complementary for melanoma treatment and prevention