ABSTRACT
Background: Coronary artery disease [CAD] is a multifactorial and heterogenic disease. Recently, genome-wide association studies have reported that rs1333040 [C/T] and rs1004638 [A/T] single nucleotide polymorphisms [SNPs] in the 9p21 locus have very strong association with CAD. This study aimed to examine these associations in Southwest of Iran
Methods: Blood samples were collected from 200 CAD patients and 110 healthy individuals with no CAD. The association of two SNPs with CAD was evaluated by PCR and restriction fragment length polymorphism
Results: Chi-square test showed no association between rs1333040 SNP and CAD [X[2]: 4.66, df: 2, P=0.09]. Also, there was no association between rs1004638 SNP and CAD [X[2]: 0.27, df: 2, P=0.88]
Conclusion: No association was observed between rs1333040 and rs1004638 SNPs in the 9P21 region and CAD in Southwest of Iran
ABSTRACT
Giardia duodenalis is one of the most important human enteric parasites throughout the world. Clinical symptoms of this parasite vary from asymptomatic infection to chronic diarrhea. Still it is not clear, whether different types of pathogenesis are due to different strains of organism or to variable host factors. The purpose of this study was to investigate possible correlation of clinical symptoms with assemblages among symptomatic and asymptomatic cases collected from southwest of Iran. Fecal samples were collected from 100 symptomatic and asymptomatic cases, which were positive for G. duodenalis. The samples were subjected to semi-nested PCR and RFLP for gdh gene. Among symptomatic patients, 54% had mixed genotypes AII and BIII, 28% and 18% of samples indicated assemblages BIII and AII, respectively. In contrast, among asymptomatic cases, 64%, 26% and 10%samples had mixed genotypes, BIII and AII assemblages, respectively. Statistical analysis using Chi-Square test showed that there was no significant correlation between assemblage and clinical symptoms in current study. High prevalence of mixed infection in both groups may affect this conclusion, therefore further study in more details are necessary to clarify these finding. Additionally, it is important to carry out investigations regarding human host factors as well
ABSTRACT
The purpose of this study was laboratory comparison of microhardness values in one kind of composite core at complete building tooth with incremental and Bulk placement techniques. Ten cylindrical samples of clearfil photo core-light cure [Kuraray/Japan], [Translucent, Single Shade] composite were prepared up to 9mm in Insulin syringes. Half of them were built with incremental placement technique by 2 mm increments and the other half were built with bulk placement technique. Light curing was done with output irradiance of 650 mw/cm2 for 40 seconds. Samples were cut in longitudinal position and were mounted in Epoxy resin. Vicker's microhardness was measured from surface of every samples in the 1,2,3,4,5,6,7,8,9 mm depths. Statistical Analyses were done with two-way ANOVA [P<0.05]. The micro hardness values of equivalent depths in bulk placement technique were more than incremental placement. There were not significant diffenences among the hardness of 1 to 9 mm depths, in two placement techniques. In order to achieve the suitable curing depth in every composite with regard to its composition and color, we should follow the light curing standard conditions of that special composite