Isolation and properties of lactate dehydrogenase isoenzyme from buffalo liver: Application in AST and ALT assay diagnostic kits

Lactate dehydrogenase (LDH) enzyme is a major component of aspartate aminotransferase (AST) and alanineaminotransferase (ALT) diagnosis kits. In this work, the LDH enzyme was purified and characterized from buffalo liverfor direct application in the preparation of AST and ALT diagnosis kits. One major LDH (BLLDH) isoform and twoother secondary LDH peaks were analyzed for buffalo liver by diethylaminoethyl (DEAE) cellulose chromatography.BLLDH was obtained by ammonium sulfate sedimentation and chromatographically separated on ion exchange andsize-exclusion matrices. The isolated BLLDH has a specific activity of 17.6 units/mg proteins represented 16 foldsand 32% recovery. BLLDH was manifested homogeneous on native and SDS gels with 35 kDa native mass. OptimumpH of BLLDH was displayed at pH 7.6. BLLDH activity was diminished by FeCl2 and SDS. The produced BLLDH isutilized in constructing of AST and ALT diagnosis kits that were sensible and analogous to trade ready kits.

influence of 50 Hz magnetic field on liver oxidative

The influence of static magnetic fields [SMFs] or modulated magnetic fields [MMFs] on biological systems has been a topic of considerable interest for many years. At the current state of knowledge, the biological effects, both in vivo and in vitro, of SMFs have yet to be unequivocally interpreted. Induced electric currents, unlike time-varying electromagnetic fields, are not associated with these fields except during activation and deactivation or when there is movement within the field. The study involved the effect of magnetic field on the oxidative state of the liver tissue. The study was conducted on Sparague-Dawely male rats of an average weight of 140-160 gm. The exposure period was 7 days [2 hours/day]. The applied field also caused an increase in the lipid peroxide [H2O2] and a decrease [p<0.05] in its glutathione [GSH] concentration. Results, as the spectroscopic examination of the serum illustrates, suggest that the redox [reduction/oxidation reaction] potential of NADH/NAD is disturbed as a result of exposure to magnetic field resulting in an exhaustion of NADH. Consequently, precursors of NADH, such as kynurenine, tyrosine and tryptophan, are supplied by the body

influence of 50 Hz magnetic field on liver enzymes

A number of epidemiological studies have suggested an increased risk for cancers, particularly leukemia and brain and breast cancer, with residential or occupational exposure to 50/60 Hz magnetic fields. Such as associated with the generation, distribution and utilization of electric power. However, researchers concluded that the results from such epidemiological studies have been inconsistent and contradictory and do not constitute reliable evidence of an association between magnetic field exposure and some forms of cancer as reported. The present study has been conducted to evaluate the influence of 50 Hz magnetic field on some liver enzymes tests; alanine aminotransferase [ALT], aspartate aminotransferase [AST], alkaline phosphatase [ALP], serum bilirubin, serum albumin and serum protein. The study was conducted on Sparague-Dawely male rats of an average weight of 140-160 gm. The exposure period was 7 days [2 hours/day]. The results showed an increase [p<0.05] in all investigated liver enzymes