ABSTRACT
Background: Studies of oxidative status in polycystic ovarian syndrome [PCOS] patients are limited with inconsistent results. The effects of resveratrol as a natural antioxidant on oxidative status in PCOS aren't clear
Objective: This study evaluated effects of resveratrol on oxidative stress in the liver and serum of the PCOS rats
Materials and Methods: Fifteen female Wistar rats [3 wk old] were divided into 3 groups [n=5/each e]: Control group, PCO-Control group, and PCO-Resveratrol group. For induction of polycystic ovary phenotype, testosterone enanthate 10 mg/kg was injected for 35 days subcutaneously. Then, resveratrol 10 mg/kg was injected intraperitoneally for 28 days to rats of the PCO-Resveratrol group. Ovarian sections were stained with hematoxylin/eosin. The serum glucose and insulin and the levels of malondialdehyde [MDA] and total antioxidant capacity [TAC] in serum and liver were measured
Results: Control animals showed normal ovarian morphology and PCO-Control animals exhibited cystic follicles. There were no significant differences in liver TAC between groups. The serum MDA [p=0.034], and homeostatic model assessment insulin resistance [HOMA-IR] [p=0.014] levels in PCO-Control rats were higher than the controls. The liver MDA in PCO-Control rats was more than that of controls [p=0.001]. The HOMA-IR [p=0.008] and serum MDA [p=0.006] levels in PCO-Control rats were more than those of PCO-Resveratrol rats [p=0.008]. In PCO-Resveratrol group, serum TAC was higher than that of PCO-Control group [p=0.022] and liver MDA was more than controls [p=0.01]
Conclusion: Results indicated that the induction of PCOS in rats increased lipid peroxidation and insulin resistance and resveratrol improved these complications
ABSTRACT
Background: One of the problems that addicts suffer from is decreased libido. Erectile dysfunction has been reported in men using opioids for treatment of heroin addiction. Objective: The study was performed to investigate the effects of morphine and detoxification with methadone as causes of sexual dysfunction in addiction. Methods and Methods: A total of 40 adult male rats [Wistar] were used. Animals were divided in to 4 groups. Control groups received saline for 30 days. Other 2 groups received 10 mg/kg morphine on day 1 and the morphine doses increased daily by 2 mg/kg increments per day until in day 30 a maximum of 68 mg/kg twice daily was achieved. Withdrawal syndrome sings were evaluated. At the end of period, one group of 2 morphine dependent groups was treated with methadone during 14 days. Animals in group 4 [saline solution+ methadone] received saline for 30 consecutive days and then detoxified with methadone during 14 days. Partial weights of seminal vesicles, testes, prostates, seminal vesicles content, concentrations of luteinizing hormone, follicle stimulating hormone, and testosterone in serum were determined. Results: In the dependent group serum levels of testosterone [p<0.001], folicle stimulating hormone [p=0.0097] and luteinizing hormone [p=0.0031] as well as the weights of testes [p=0.0051], partial weights of prostates, seminal vesicles and seminal vesicles contents [p<0.001] were reduced as compared with control group. In the morphine dependent animals detoxified with methadone, testosterone concentrations and seminal vesicles contents remained lower than levels in the control group [p<0.001]. Conclusion: The results suggest that morphine dependence may impair the reproductive function in male rats