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Objective To perform Quality comparative study of urine visible components tests by using Sysmex UF-1000i auto-mated urine analyzer (Sysmex UF-1000i) ,AX-4030 automated urine chemistry analyzer (AX-4030) ,and three kinds of manual opti-cal microscope instrument .Methods Applying the above three kinds of instrumental methods respectively to 2 086 case of RBC , WBC and the CAST test and their positive rate and sensitivity difference were analyzed .Results SysmexUF-1000i ,AX-4030 and microscopic three kinds of instrument detection RBC ,WBC ,CAST positive rates were as follows ,Sysmex UF-1000i:20 .4% 、20 .8% 、6 .4% ;AX-4030 :23 .6% 、16 .5% (no CAST );microscope:15 .2% 、18 .6% 、2 .4% .Among the 3 kinds of tests ,RBC ,CAST detection rate were significantly different (P0 .05) .Conclusion Sysmex UF-1000i and AX-4030 can not completely replace the gold standard manual microscopic examination of urine sediment should be three kinds of methods used in combination to ensure the accuracy of the urine analysis results .
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With widely application of bronchoavleolar lavage , a lot of cases on L.Blattarum infections in respiratory system have emerged in recent years .However , after closely lucubrating pictures of these reported cases and analyzing the results of our animal experiments.It was doubted the reported morphology of L.Blattarum belongs to respiratory ciliated columnar epithelium .This article aimed at guiding our colleagues to distinguish the morphology of Lophomonas blattarum in respiratory system, and,avoid the misdiagnosis.
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Objective To investigate the long-term excessive drinking semen nitric oxide (NO) content in their sperm quality and spermatogenic cell apoptosis and infertility. Methods Nitrate reductase method was used to specific reduce the NO metabolites nitrate (NO3-) to nitrite (NO2-), which was used on behalf of the total NO level. Terminal deoxynucleotidyl transferase (TdT) and mediated nick end labeling (TUNEL) method and binocular optical microscope were used to detect and observe the rate of apoptosis of spermatogenic cells and the morphological structure. The SQA-V sperm quality automatic analyzer was used to measure sperm quality. Results In not drinking semen fertility group, NO content was (54.81±11.45)μmol / L, the rate of spermatogenic cell apoptosis was (4.52±1.23)%, sperm motility was (80.24±0.17)%, energy a+b (78.32±0.12)%, deformity rate (5.30±0.13)%, and long-term excessive alcohol infertility C group was [(128.83±22.73)μmol/L,(17.34±2.53)%,(51.18±0.58)%,(21.45±0.26)%,(21.12±3.24)%] respectively. Compared to a very significant difference (t＝10.04,17.38,6.69,15.59,17.02,P＜0.01) . In long-term excessive drinking group, the levels of NO and spermatogenesis cell apoptosis rate was significantly positive correlated (r＝0.93,P＜0.01).Apoptosis of spermatogenic cell nucleus chromatin was condensed in the formation of crescent-shaped perinuclear, nuclear was cleavaged to form apoptotic bodies. Conclusions In the long-term excessive drinking semen, NO content and spermatogenic cell apoptosis rate was increased, the sperm had poor quality. The results showed that the long-term excessive drinking can cause germ cell apoptosis in male infertility and promote the body to overproduce NO, whichmay be one of the reasons for male infertility.
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Objective To investigate the relationship between clue cell in semen and male infertility. Method Semen specimens from 957 patients were examined with microscope. Gram staining was performed when clue cell were found to be present in semen. At the same time, general characteristics of the sperm were analyzed. Results There were clue cells in 23. 5% (225/957) of the total specimens, in which, 95. 1% clue cells were gram staining positive. The concentration and vitality of living sperm in semen presented with clue cell were significantly lower, but the abnormal sperm and pH value were much higher than that in normal control (P<0.01). In addition, 28 sexual partners of 30 infertility patients were identified as having bacterial vaginosis. Conclusion Gardnerella vaginalis and short coccohacillus were mostly transmitted from infected sexual partner. It could cause squamous epithelial cell forming clue cell, and lead to infertility by chan-ging pH which mainly affect reproduction of the sperm. This study suggested that clue cell has a unique vale for the diagnosis of infertility causing by Gardnerella vaginalis and short coccohacillus.
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Objective To purify Micrococcus luteus Rpf and Rpf domain fusion protein, and to in-vestigate its effects on growth of Mycobacterium tuberculosis. Methods The recombinant plasmids pPro-EXHT-Rpf and pPro-EXHT-Rpf domain were expressed in E. Coli DHSa and then purified under denaturing condition via Ni-NTA purification system and confirmed by Western blot. The biochemical property of the M. Luteus Rpf and Rpf domain was analyzed by stimulating the resuscitation of M. Tuberculosis H37Ra which were in non-culturable' condition. Results The Rpf and Rpf domain products achieved 95% and 93% pure respectively, and the molecular weight was 30 x 103 and 12 x 103, the yield of purification was about 471 mg/L and 337 mg/L of the culture. The M. Luteus Rpf and Rpf domain from the E. Coli showed activity of stimulating the resuscitation of M. Luteus and M. Tuberculosis H37Ra in non-cuhurable' condition which could be inhibited by monoclonal antibodies of M. Luteus Rpf domain remarkably. Conclusion It was dem-onstrated that the purification of Rpf and Rpf domain have high biological activity for further functional, pharmacological and clinical investigations, and M. Luteus Rpf domain protein is fully active as M. Lateus full-length Rpf.
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<p><b>OBJECTIVE</b>To study the function of zinc in preventing human sperm from being damaged by sodium nitroprusside (SNP), an external NO donor.</p><p><b>METHODS</b>Analyses were made of the function of zinc in protecting sperm from being influenced by SNP in such aspects as sperm motility, head-tail connection and the breakage of sperm DNA chain by using phase-contrast microscope and single cell gel electrophoresis (SCGE).</p><p><b>RESULTS</b>Sperm motility was obviously inhibited by SNP. The percentage of comet cells increased significantly but the stability of sperm head-tail connection decreased. Zinc could promote sperm motility, protect the DNA chain and prevent the sperm head-tail connection from breaking.</p><p><b>CONCLUSION</b>Zinc can protect sperm from being damaged by NO. Its mechanism may be related to the mercaptol group of sperm chromatin.</p>