IL-10 and IL-12 production in response to mycobacterium tuberculosis total lipid antigens in multidrug-resistant tuberculosis

Mycobacterium tuberculosis lipid antigens take part in pathogenicity of the bacterium but the response of monocytes/macrophages to these antigens in tuberculosis is not well known. The aim of current investigation was to study the M. tuberculosis lipid antigens in tuberculosis pathogenesis. In the present study M. tuberculosis lipid antigens were extracted. Monocytes and macrophages from multidrug-resistant tuberculosis [MDR-TB], TB patients, asymptomatic healthy individuals with positive tuberculin skin test positive and healthy individuals with negative tuberculin skin test were collected using magnetic cell sorting. The cells were stimulated by M. tuberculosis total lipid antigens and IL-12 and IL-10 in their supernatants were measured by enzyme-linked immunosorbent assay. The IL-12 production by monocytes in response to M. tuberculosis total sonicate antigens in the MDR-TB patients did not show a considerable difference with the PPD positive healthy subjects, whereas in the active TB patients, IL-12 levels significantly decreased [p<0.05]. IL-10 production by monocytes in TB patients in response to total lipid antigens showed a significant increase in comparison to MDR-TB patients and healthy individuals. In the MDR-TB patients, IL-10 and IL-12 production by monocytes in response to M. tuberculosis lipid antigens are similar to the healthy subjects

Immune Responses of CD4 [+] T-cells of MDR-TB patients to M. tuberculosis total lipid antigens

CD4 [+]T-cell have a central role in protective immune responses to Mycobacterium tuberculosis [M. tuberculosis] protein antigens, but function of these cells in response to M. tuberculosis total lipid antigens has remained unclear. The present study was undertaken to determine role of CD4[+] T cells in the MDR-TB patients against M. tuberculosis total lipid antigens. CD4[+]T- cells were isolated from MDR-TB patients and stimulated with M. tuberculosis total lipid antigens. Proliferative responses and cytokine secretion were assessed by MTT and ELISA, respectively. Our study results showed that proliferative responses of stimulated CD4[+]T- cells to M. tuberculosis total lipid antigens and IFN-gamma production in MDR-TB patients were significantly lower than those of the PPD-positive subjects [P < 0.05] whereas, IL-4 production in the MDR-TB patients was elevated[P < 0.05]. Responses of CD4[+]T -cells of MDR-TB patients to total lipid antigens was significantly lower than that of PPDpositive healthy subjects. Therefore, it seems that M. tuberculosis lipid antigens, as protein antigens, have an important role in specific immune response