ABSTRACT
The present study estimated cytokinin-like activity [CLA] in blood and urine samples collected from 15 patients with chronic renal failure [CRF] and 10 normal controls. CLA was determined using one of the bioassay technique which is the Cucumber seeds test after fractionation of serum and urine with ethyl acetate and n. butanol. All urine samples were fractionated and bioassayed after being bacteriologically filtered to avoid the interference with free cytokinin owned by bacteria. Our results showed that the values of CLA were significantly reduced in the blood and urine specimens of patients with CRF versus that of control group [P for both were< 0.05]. This preliminary work, which is done for the first time, may suggest studying the identification, metabolism, biosynthesis and turn-over of the substance [S] which is present in human being and exerts the biological activity of cytokinin