ABSTRACT
Effects of uterine stretching and physiological hypertrophy on myosin isozyme were investigated in rat during pregnancy. Both nonpregnant and pregnant rat uteri express a single myosin band on native gels. Analysis of native myosin under denaturing conditions revealed two myosin heavy chains (MHCs) with molecular mass of 204 and 200 kDa respectively. Filamin, a 240 kDa protein co-electrophoreses with myosin on native gels. No correlation is found between regulatory myosin light chain phosphorylation and pattern of myosin isozymes or the MHC. The results suggest that uterine stretching and physiological hypertrophy during pregnancy do not induce any changes in uterine myosin isozyme.
Subject(s)
Animals , Female , Muscle, Smooth/enzymology , Myosins/isolation & purification , Pregnancy , Pregnancy, Animal/metabolism , Rats , Rats, Wistar , Uterus/enzymologyABSTRACT
Myosin light chains in normal and minced autografted chick gastrocnemii have been studied by disc gel electrophoresis during 70 days postgrafting period. As a result of induced mechanical trauma, the quantitative relationships between myosin light chain fractions are disturbed affecting the muscle functions.
Subject(s)
Animals , Chickens , Male , Muscle, Skeletal/chemistry , Myosin Light Chains/analysis , Regeneration/physiology , Transplantation, AutologousABSTRACT
A comparative study of myosin light chains (MLCs) has been made in the aorta, uterine and cardiac muscles (auricle, ventricle) of mice, pig, sheep and goat. Analysis of myosin light chains by sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE) has revealed that (a) aorta myosin from mice, goat and pig has identical myosin light chains profile but pig aorta myosin lacks LC-2f; (b) uterine smooth muscle myosin depicts absence of LC-1f in sheep. Whereas satellite bands of LC-1f and LC-2f fractions are absent in pig uterine myosin, mice shows duplets for both the myosin light chains; (c) the auricular myosin in pig and goat is identical to chicken gizzard myosin used as reference and exhibits ALC-2s and ALC-1s fractions only while sheep auricular myosin lacks in ALC-1f; (d) the mice ventricular myosin depicts two satellite MLCs associated with fast migrating VLC-1s.
Subject(s)
Animals , Aorta/metabolism , Chickens , Electrophoresis, Polyacrylamide Gel , Female , Goats , Mice , Molecular Weight , Muscle, Smooth/chemistry , Myocardium/chemistry , Myofibrils/metabolism , Myosin Light Chains/chemistry , Sheep , Swine , Uterus/chemistryABSTRACT
Changes in the specific conductivity and electrolytes have been studied in the leachates from normal and minced autografted chick gastrocnemii with special reference to the loss of Na+, K+, Ca2+, Mg2+ and PO(4)3- ions. The study has revealed age related modifications in the muscle membranes as well as the presence of some intrinsic regulatory mechanisms which get stimulated under the induced-stress condition. Higher ionic efflux in the regenerating muscle indicates that the anatomical regeneration does not correspond to functional regeneration of muscle membranes.
Subject(s)
Animals , Chickens , Electrolytes/metabolism , Muscles/metabolism , RegenerationABSTRACT
Biochemical estimation of acidic and basic proteins of chick gastrocnemii (G. externus, G. medius and G. internus) and pectoralis muscles has been done under normal, denervated and work stress conditions from 1-56 days of postnatal growth. The reciprocal relationship of the two protein groups is clearly established. It is evident that muscle denervation acts as a stimulant for proteosynthetic activities and probably may also be an inhibitory factor for protein degradative reactions. During work overload stress, the rapid growth of muscles has been related to high rate of contractile activity.
Subject(s)
Animals , Chickens/metabolism , Hydrogen-Ion Concentration , Muscle Denervation , Muscle Proteins/analysis , Muscles/chemistry , Stress, Physiological/metabolism , Weight-BearingABSTRACT
White leghorn male chicks of 1 and 7 day age groups were studied for acute (2.25 Gy) gamma radiation (with or without vit. E pretreatment) induced haematological changes in the peripheral blood at days 1, 3, 5, 7, 14 and 28 postirradiation. A continuous decrease in the erythrocyte numbers was observed in the animals irradiated without vit. E treatment. The changes in haematocrit, haemoglobin, MCV, MCH and MCHC values were in line with the erythrocytic changes reflecting radiation induced damage to the erythroid elements. Animals pretreated with vit. E show lesser depression in the erythrocytic component at all the stages indicating its radio-protective influence. The significant increase in the immature RBC's in the peripheral blood in vit. E treated animals after irradiation, implies enhanced erythropoiesis.
Subject(s)
Animals , Chickens , Erythrocytes/drug effects , Gamma Rays/adverse effects , Male , Radiation, Ionizing , Time Factors , Vitamin E/pharmacologyABSTRACT
Newly hatched white leghorn chicks (Gallus domesticus) subjected to single whole body 2.25 Gy (225 rads) gamma radiation exposure at the dose rate of 0.50 Gy/sec (50 rads/sec), were studied for changes in a number of haematological parameters at days 1,3,5,7,14 and 28 post irradiation during development. The sudden decline and gradual recovery in total RBC and WBC counts and the level of Hb and Hct along with MCV, MCH and MCHC values evaluation indicates a high regenerative capability of leghorn chicks.
Subject(s)
Animals , Blood/radiation effects , Chickens , Cobalt Radioisotopes , Gamma Rays , MaleABSTRACT
Leaching behaviour of chick gastrocnemius muscle has been studied with respect to the loss of Na+, K+ and Ca2+ ions in a donor-solvent (muscle-water) system under normal as well as denervated conditions. An attempt has been made to explain the alterations in the rate of electrolytic loss in terms of membrane dysfunctions induced as a result of loss of neural control.
Subject(s)
Animals , Calcium/metabolism , Chickens , Muscle Denervation , Muscles/innervation , Potassium/metabolism , Sodium/metabolismABSTRACT
Alterations in the phospholipid levels of three gastrocnemii (G. externus, G. medius and G. internus) of chick have been studied during 56 days postembryonic growth of the three muscles. The effects of denervation and work-overload stress on their phospholipid content during the same period have been discussed in the light of denervation-induced membrane breakdown and exercise-induced fibre hypertrophy.
Subject(s)
Animals , Chickens , Muscle Denervation , Muscles/metabolism , Phospholipids/metabolism , Stress, PhysiologicalABSTRACT
The leaching of electrolytes in normal and denervated chick gastrocnemius muscle has been studied by recording electroconductivity changes in donor-solvent (muscle-water) system at 11 intervals leading to a total immersion of 5 hr 15 min, at days 1,5,10,21 and 28, post-hatching. The results show age related changes in the permeability properties of normal muscle membrane system. The loss of neural control induces a blocking of electrolytes 10 days post-denervation. The possible nature of blocking mechanism has been related to the non-availability of neuro-trophic factors in the tissue.