In vitro cytotoxic effect of urtica dioica extracts on acute myelogenous leukemia cell line [kg-1]

Background: Urtica dioica is one of the medicinal herbs with many uses in treating various diseases. In some studies, its antiproliferative and apoptotic effects on cancer cell lines have been shown. Therefore, the evaluation of U. dioica effect was performed on KG-1 cell line for acute myelogenous leukemia [AML] for the first time in this study

Materials and Methods: KG-1 cell line was treated by various extracts [aqueous, hydroalcoholic, chloroform and ethyl acetate] of U. dioica aerial parts and roots in different concentrations. Metabolic activity of extracts on cell line was assessed by MTT assay. To evaluate the percentage of apoptotic cells, the flow cytometry was performed by FITC Annexin V-PI apoptosis detection kit in KG-1 cell line treated with root chloroform [UDC-R] and ethyl acetate [UDE-R] extracts. The results have been reported as percentage of cell viability and IC50

Results: Based on MTT results, the strongest IC50 in KG-1 cell line [219.361microg/ml] was related to UDC-R. The flow cytometric analysis showed that UDC-R and UDE-R in IC50 concentration induced early [53.6% and 57.4%, respectively] and late [27% and 33.2%, respectively] apoptosis in KG-1 cells after 24 hrs. The inhibition of cell proliferation by various extracts of U. dioica was dependent on concentration [p=0.000]

Conclusion: Flow cytometric analysis confirmed that UDC-R and UDE-R extracts affect on proliferation reduction of KG-1 cells by activating the apoptotic pathway

2-Amino-4-[nitroalkyl]-4H-chromene-3-carbonitriles as new cytotoxic agents

A series of 2-amino-4-[nitroalkyl]-4/-chromene-3-carbonitriles were synthesized by an efficient multicomponent reaction in aqueous media using DBU as a catalyst at room temperature. Mild condition, environment friendly procedure and excellent yields are the main advantages of this procedure. The cytotoxic activity of target compounds were evaluated against three cancer cell lines MDA-MB-231, MCF-7 and T47D in comparison with etoposide as reference drug. Generally, all compounds showed good cell growth inhibitory activity with IC[50] values less than 30 microg/mL. Their activities were comparable or more potent than standard drug etoposide. The 6-bromo- derivatives 7e and 7f showed promising cytotoxic activity with IC[50] values in the range of 3.46-18.76 microg/mL, being more potent than etoposide against all tested cell lines