ABSTRACT
Objective To investigate the relationships between the HPV infection and race susceptibility in the carcinogenesis of Uighur women with cervical cancer from the southern Xinjiang, one of the high risk region of cervical cancer in China. Methods To detect 21 subtypes of HPV and the 13 alleles of HLA from 200 cervical cancer cases and 200 normal tissues, by using flow-through hybridization and gene chip (HybriMax) method and polymerase chain reaction sequence-specific oligonucleotide method (PCRSSO). Results ( 1 )The proportion of HPV positive in cervical cancer and control group were 88.5% and 7.0% respecfively;HPV16 was the most common type in HPV positive cervical cancer patients with the rate of 90.96%, following were HPV18 (5.08%), HPV68(3.95% ),HPV45 (3.39%), HPV58 (3.39%),HPV39( 1.69% ), HPV31 ( 1.69% ), HPV56( 1.13% ). In cervical cancer and control group, the positive rate of HPV and HPV16 were significantly higher than that in control group. (2) In cervical cancer group the frequency of HLA-DRB1 * 15 in HPV positive cervical cancer cases was significantly higher than that among the HPV negative cases. While the frequency of HLA-DRB1 * 12 in HPV positive eervical caneer eases was significantly lower than that in the HPV negative e asea. Conclusion HPV16 was the most common type in both cervical cancer and control groups, the frequency of HPV16 in cervical carcinomas was very high, following HPV18 and HPV68, and HPV68 ranked third which was different from the results of other reports,this indicates that Uighur women are infected with this type more common. It appears that HLA-DRB1 * 15may be related to the susceptibility to cervical cancer and the HPV16 infection among the Uighur women,while the HLA-DRB1 * 12 the protective gene to HPV16 infection in Uighur women. The study of HLA alleles in the carcinogenesis of cervical carcinomas may play an important role in the gene intervention research of cervical cancer.
ABSTRACT
Purpose:To study the association between human papillomavirus (HPV) and cervical cancer in Uigur women from southern Xinjiang where there is a high risk of cervical cancer.Methods:HPV-C,HPV16,HPV18 and HPV6/ 11 were detected in 40 fresh tissues of cervical cancer and 40 tissues of normal cervix by using polymerase chain reaction technique.Results:The positive rate of HPV-C,HPV16,HPV18 and HPV6/11 in fresh tissue of cervix cancer were 87. 5%,72.5%,10.0% and 0,respectively;The positive rate of HPV-C,HPV16,HPV18 and HPV6/11 in normal cervix were20.0%,5.0%,0 and 7.5%,HPV16 was the most common type in HPV-C positive patients with the rate of 82.8 %. The positive rate of HPV-C and HPV16 in cervical cancer were significantly higher than that in control groups;The positive rate of HPV16 in squamous cancer of cervix was significantly higher than that in adenocarcinomas of cervix;while the posi- tive rate of HPV 18 in adenocarcinomas of cervix was significantly higher than that in squamous cancer of cervix(P0.05).Conclusions:HPV16 infection plays an important role in the carcinogenesis of cervical cancer of Uigur women from Southern Xinjiang,HPV16 infection is closely correlated with squamous carcinomas of cervix,while HPV18 infection is closely correlated with adenocarcinomas of cervix.The positive rate of HPV-C and HPV16 have no correlation with clinical stages and histological grade of cervical carcinomas.