ABSTRACT
Introduction: Diabetic mellitus is a complex and a diverse group of disorders that disturbs the metabolism of carbohydrates, fat and protein. Plant based medicaments are extensively used for the treatment of various ailments of human beings. The main objective of the present investigation includes the preliminary screening of phytochemicals and evaluation of in vitro antidiabetic efficacy of Physalis angulata fruit extracts. Materials and Methods: Plant material was subjected to sequential extraction by maceration method by using different solvents. Primary screening of the fruit extract was conducted for detection of various types of phytochemicals, whereas antidiabetic activities of Physalis angulata were evaluated using inhibition of alpha amylase and alpha glucosidase enzymes Results: The study reveals the presence of phytochemicals such as alkalodis, saponins, phenols etc. Among the tested extracts of fruit methanol has been successfully inhibited both of the enzymes in vitro. The highest inhibition percentage 97.23 and 96.53 noticed against alpha amylase and alpha glucosidase at 100 μg/ml respectively. The results are comparable with the percentage 95.16% recorded with reference drug acarbose at 10 μg/ml. Conclusion: From the data obtained in the current studies, it was observed that the fruit extracts of Physalis angulata showed prominent antidiabetic properties in vitro and further the studies can be carried out for isolation of active principle responsible for activity.
ABSTRACT
The plant Acmella Paniculata commonly known as Akala Kara belonging to family of Asteraceae mainly found in tropical and subtropical regions around the world. Annual herb grown upto 30 cm tall with pungent taste. All parts of this plant are being used to traditional medicine to treat various diseases. Pet ether, chloroform, ethyl acetate and methanol extracts from whole plant of Acmella Paniculata Were investigated for their antioxidant and antimicrobial activities In case of antioxidant activity methanol extracts shows 96.66% of radical scavenging activity at 100μg/ ml. In case of antimicrobial activity chloroform extracts shows 30mm zone of inhibition against the Enterobactum aurogenosa. Methanol extracts shows 25 mm zone of inhibition.
ABSTRACT
The trend of using natural products has increased and the active plant extracts are frequently screened for new drug discoveries. The present study deals with the screening of Casia occidentalis leaves for their antimicrobial activity against various strains of bacteria. Plant Cassia occidentalis belongs to family Caesalpiniaceae, is a diffuse offensively odorous under shrub. Casia occidentalis were shade dried, powered and was extracted using solvents Methanol. The antimicrobial activity test performed by the disc diffusion method. Preliminary phytochemical analysis of the plant extracts fractions of HXF, CTF, CFF and AQF showed the presence of carbohydrates, amino acids, phytosterols, fixed oils and phenolic compounds. The AQF fraction of C. occidentalis showed high activity across pseudomonas aeuruginosa and staphylococcus aureus bacteria. The present study indicates the potential usefulness of Casia occidentalis leaves in the treatment of various diseases caused by micro-organisms.
ABSTRACT
Highly active anti-retroviral therapy (HAART) is the current HIV/AIDS treatment modality. Despite the fact that HAART is very effective in suppressing HIV-1 replication and reducing the mortality of HIV/AIDS patients, it has become increasingly clear that HAART does not offer an ultimate cure to HIV/AIDS. The high cost of the HAART regimen has impeded its delivery to over 90% of the HIV/AIDS population in the world. This reality has urgently called for the need to develop inexpensive alternative anti-HIV/AIDS therapy. This need has further manifested by recent clinical trial failures in anti-HIV-1 vaccines and microbicides. . In the current study, we characterized a panel of extracts of traditional medicinal plants for their activities against HIV-1 replication. The aim of the present study was to evaluate the invitro anti- HIV activity of Eclipta alba plant extracts. Extracts were prepared from dried fruits in n-hexane, ethyl acetate and n butanol. Peripheral Blood Mononuclear Cells (PBMCs) isolated from healthy donors by ficoll-hypaque density gradient centrifugation method. A toxicity study was performed on all crude extracts by MTT assay using PBMCs isolated from whole blood. HIV-1 RT inhibition activity of the all solvent extracts of Eclipta alba was determined by a RetrsoSys HIV-1 RT activity kit (Innovagen, Sweden). The aerial parts of Eclipta alba extracts are shows anti-HIV-1 activity and this plant has great potential for developing useful drugs.