ABSTRACT
Aim of the work: This work aimed to study the biochemical and histopathological changes in the kidney of male albino rats post exposure to 6Gy of gamma radiation and the protective role of transplanted bone marrow cells against damage induced in rat's kidney by a chemical carcinogen
Materials and Methods: In this study, forty eight healthy and active male albino rats about 120 grams in body weight were used. The animals were housed in plastic cages under normal temperature, pressure, humidity and good ventilation conditions during the whole period of experimentation. The animals were fed on a standard pellet diet and water
Results: Exposure of rats to gamma-radiation caused a significant increase in kidney function tests, decreased significantly the antioxidants with numerous histopathological changes in the rat kidney tissue. These changes were ameliorated by bone marrow transplantation either after whole body gamma-irradiation and/or Fe-NTA treatment
Conclusion: Bone marrow transplantation either after whole body gamma-irradiation and/or Fe-NTA treatment restored the kidney functions and ameliorated the oxidative stress and antioxidants markers. The histopathological observations showed amelioration in the structure of the kidney cortex. So, BM transplantation exerts some curative effects on the function and histological structure of kidney cortex of rats exposed to gamma-irradiation and/or Fe-NTA treatment
ABSTRACT
Aim of work: this work aimed to study the biochemical and histopathological changes in the liver of male albino rats post exposure to 6Gy of gamma radiation and the possible protective effect of bone marrow [BM] transplantation on the liver tissues by a chemical carcinogen ferric nitrilotriacetate [Fe-NTA] or gamma- radiation in rats
Materials and methods: In this study, thirty six healthy and active male albino rats about 120 grams in body weight were used. The animals were housed in plastic cages under normal temperature, pressure, humidity and good ventilation conditions during the whole period of the experiment. The animals were fed on a standard pellet diet and water. Animals were categorized into six groups and served as the following groups: control, gamma irradiated[R], Fe-NTA, BM+R, BM + Fe-NTA and BM.+Fe-NTA+R
Results: the present results suggested that exposure to gamma-radiation or Fe-NTA induced a significantly disturbance in the liver functions and structure. They increased significantly the oxidative stress and decreased significantly the antioxidants tissues and they also increased necrotic and apoptotic cells in rat's liver tissue. Bone marrow transplantation either after whole body gamma-irradiation or Fe-NTA treatment restored the liver functions and structure. BT also ameliorated the oxidative stress and antioxidative markers. The histopathological observations recorded some amelioration in the apoptotic and necrotic evaluation in liver tissue
ABSTRACT
Antibodies directed to citrulline-containing proteins have been shown to be specific for rheumatoid arthritis [RA]. They may be related to disease activity and extent of joint damage. To investigate the value of anti-citrullinated cyclic peptide antibodies [anti-CCP] in diagnosis and clinical evaluation of RA patients compared with rheumatoid factor [RF] isotypes [IgG-, IgA- and IgM-RF. In a cross-sectional study, anti-CCP and RF isotypes were assayed by ELISA in 44 RA patients, 25 patients with arthritis other than RA and 15 healthy controls. RA disease activity was assessed as mild [18 patients], moderate [15 patients] or severe [11 patients] using DAS 28 score. Bone involvement in RA was classified radiologically [recent radiographs for hands and wrists] into 3 categories: no bone involvement [16 patients], juxta-articular osteoporosis [13 patients] or joint erosions [15 patients]. According to disease duration, those with duration less than one year were grouped as early RA [17 patients] and those with duration more than one year as established RA [27 patients]. Sensitivity of anti-CCP was 72.7%; non significantly different [p>0.05] from those of IgM-RF [63.6%] or cumulative RF isotypes [75%] and significantly higher [p<0.05] than IgA- [47.7%] and IgG-RF [36.4%]. Specificity of anti-CCP was 100%; significantly higher [p<0.05] than those of IgA- [92.5%], IgG- [87.5%], IgM-RF [80%] and cumulative RF isotypes [70%]. Positive predictive value of anti-CCP was 100%; significantly higher [p<0.05] than those of IgA- [87.5%], IgM-[77.8%], IgG-RF [76.2%] and cumulative RF isotypes [73.3%]. Negative predictive value of anti-CCP was 76.9%; non significantly different [p>0.05] from those of cumulative RF isotypes [71.8%] or IgM-RF [66.7%] and significantly higher [p<0.05] than those of IgA- [61.7%] and IgG-RF [55.6%]. Anti-CCP positivity in RA patients with bone erosions was 100%; significantly higher [p<0.05] than those of cumulative RF istypes [93.3%], IgM- [86.7%], IgA- [86.7%] and IgG-RF [46.7%]. Anti-CCP positivity in patients with severe disease activity was 90.9%; non significantly higher [p>0.05] than cumulative RF isotypes [81.8%] and significantly higher [p<0.05] than those of IgA- [72.7%], IgM- [54.5%] and IgG-RF [27.3%]. Anti-CCP and RF isotypes positivity in early RA were non significantly different from those in established RA. Of special diagnostic value was the detection of positive anti-CCP in 72.2% [8/11] of RA patients in whom all RF isotypes were negative. This was especially signified in RA patients with bone erosions, severe disease activity or established. Anti-CCP proved to be a powerful diagnostic tool in RA especially in RF negative patients. It can be potentially useful as prognostic index for bone involvement and disease activity