ABSTRACT
Hemolysin [amboceptor] which is the most important constituent in complement fixation test [CFT] was prepared by inoculation of sheep RBCs S/C in Bosket rabbits in two concentrations [10% and 20%]. Blood samples were obtained weekly from inoculated rabbits to measure the titre of induced Hemolysin by CFT. It was found that Hemolysin titer was increased with the number of inoculation till the 7[th] inoculation then began to decrease with the 8[th] inoculation. However the titer of Hemolysin obtained from rabbits inoculated with 20% sheep RBCs was higher [1/5800] than that obtained from rabbits inoculated with 10% red cell suspension [1/4500]. Similar results were reported to the imported Hemolysin. By using the prepared Hemolysin in CFT for diagnosis of FMD and Brucellosis it gave the same results as that obtained by imported one